Using EGFP as reporter for the construction of expression vectors carrying structural/non-structural genes from dengue virus type II strain PL046

• Main Author: 洪禎憶
• Other Authors: 楊昀良
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/33481458022584483928

碩士 === 國立交通大學 === 生物科技學系 === 100 === Dengue virus is a single-stranded, positive-sense RNA virus with 10.7 kb genome , which can stimultaneously serves as an mRNA for translation of the viral proteins which will be digested by enzymes into three structural(C,prM,E) and seven nonstructural proteins(NS1,NS2A,NS2B,NS3,NS4A,NS4B,NS5). In this study , I constructed plasmids which contained the structural and nonstructural genes separately along with an EGFP as the reporter, for detecting the packaging signal. Next, I sub-cloned a full-length E gene from Dengue virus type II PL046 strain into pLP vector to express the E proteins for functional study. These constructs were assessed by restriction digestion and sequencing. No non-sense mutation was detected in the coding regions.