PDMS-based cylindrical hybrid lens for fluorescence enhancement used in microfluidics

• Main Authors: Yang, Han-Yu, 楊翰宇
• Other Authors: Lin, Bor-Shyh
• Format: Others
• Language: en_US
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/91557050927847378187

碩士 === 國立交通大學 === 影像與生醫光電研究所 === 100 === Microfluidic systems have recently been developed and applied for many biological and chemical applications based on fluorescence detections. Because of the tiny amount of sample in the system, the induced fluorescence can be weak. Therefore, most of microfluidic systems require many optical components, such as filters, lens, or micro structure, to enhance the efficiency of fluorescence detection. However, these components encounter the common issues of complex manufacturing processes and expensive costs. In this study, a miniature, cylindrical, and hybrid lens made of polydimethylsiloxane (PDMS) is proposed to focus the excitation light and simultaneously improve the efficiency of collecting fluorescence in the microfluidic device. The hybrid lens integrated a laser focusing lens and a fluorescence collecting lens to achieve the dual functions and simplify the optical setups. Moreover, PDMS has advantages of low-cost and easy fabrication compared with conventional optical components. For the efficiency of focusing excitation light, the proposed hybrid lens can provide an 81.7% reduction of spot size and 93.39% efficiency enhancement of the excitation light. For the integrated efficiency of collecting fluorescence, the proposed hybrid lens can provide 85.00% and 108.42% fluorescence enhancements for Rhodamine 6G and Nile Red respectively.