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• Main Authors: Wei-Hung Liu, 劉威宏
• Other Authors: Chih-ang Chung
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/37264848055131527200

碩士 === 國立中央大學 === 生物醫學工程研究所 === 100 === Many research results have demonstrated that stem cells can differentiate into specialized cells by chemical induction. Physical stimuli have also been confirmed to be able to trigger specific gene expressions, induce secretion of proteins and modulate cell behaviors such as growth, migration, proliferation and differentiation. In this study we designed a cone-plate bioreactor which can produce quantified fluid shear stress. By rotating the cone, the bioreactor drives the culture medium to flow in a circular way, which generates virtually uniform azimuthal shear stress that can be applied to the cultured cells on the plate. In order to perform a real time monitoring over the magnitudes of shear stress, a torque sensor was installed to measure the torque caused by the shear stress on the plate. After biocompatibility tests, the Placenta Derived Multipotent Cells (PDMCs) were cultured in the bioreactor. The PDMCs were then induced toward neuronal differentiation by applying both the chemical and physical stimulation simultaneously. We applied 1-methyl-3-isobutylxanthine (IBMX) as the chemical agent and investigated the effectiveness of the shear stress on cell differentiation. The application of shear stress along without IBMX addition showed no cell differentiation. However, the PDMCs differentiation initiated by the IBMX were enhanced by simultaneously applying the stress.