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碩士 === 國立中央大學 === 生物醫學工程研究所 === 100 === Many research results have demonstrated that stem cells can differentiate into specialized cells by chemical induction. Physical stimuli have also been confirmed to be able to trigger specific gene expressions, induce secretion of proteins and modulate cell be...
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ndltd-TW-100NCU051140032015-10-13T21:22:21Z http://ndltd.ncl.edu.tw/handle/37264848055131527200 none 流體剪應力結合1-甲基-3-異丁基黃嘌呤對於人類胎盤幹細胞分化之影響 Wei-Hung Liu 劉威宏 碩士 國立中央大學 生物醫學工程研究所 100 Many research results have demonstrated that stem cells can differentiate into specialized cells by chemical induction. Physical stimuli have also been confirmed to be able to trigger specific gene expressions, induce secretion of proteins and modulate cell behaviors such as growth, migration, proliferation and differentiation. In this study we designed a cone-plate bioreactor which can produce quantified fluid shear stress. By rotating the cone, the bioreactor drives the culture medium to flow in a circular way, which generates virtually uniform azimuthal shear stress that can be applied to the cultured cells on the plate. In order to perform a real time monitoring over the magnitudes of shear stress, a torque sensor was installed to measure the torque caused by the shear stress on the plate. After biocompatibility tests, the Placenta Derived Multipotent Cells (PDMCs) were cultured in the bioreactor. The PDMCs were then induced toward neuronal differentiation by applying both the chemical and physical stimulation simultaneously. We applied 1-methyl-3-isobutylxanthine (IBMX) as the chemical agent and investigated the effectiveness of the shear stress on cell differentiation. The application of shear stress along without IBMX addition showed no cell differentiation. However, the PDMCs differentiation initiated by the IBMX were enhanced by simultaneously applying the stress. Chih-ang Chung 鍾志昂 2012 學位論文 ; thesis 81 zh-TW |
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碩士 === 國立中央大學 === 生物醫學工程研究所 === 100 === Many research results have demonstrated that stem cells can differentiate into specialized cells by chemical induction. Physical stimuli have also been confirmed to be able to trigger specific gene expressions, induce secretion of proteins and modulate cell behaviors such as growth, migration, proliferation and differentiation.
In this study we designed a cone-plate bioreactor which can produce quantified fluid shear stress. By rotating the cone, the bioreactor drives the culture medium to flow in a circular way, which generates virtually uniform azimuthal shear stress that can be applied to the cultured cells on the plate. In order to perform a real time monitoring over the magnitudes of shear stress, a torque sensor was installed to measure the torque caused by the shear stress on the plate.
After biocompatibility tests, the Placenta Derived Multipotent Cells (PDMCs) were cultured in the bioreactor. The PDMCs were then induced toward neuronal differentiation by applying both the chemical and physical stimulation simultaneously. We applied 1-methyl-3-isobutylxanthine (IBMX) as the chemical agent and investigated the effectiveness of the shear stress on cell differentiation. The application of shear stress along without IBMX addition showed no cell differentiation. However, the PDMCs differentiation initiated by the IBMX were enhanced by simultaneously applying the stress.
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Chih-ang Chung |
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Chih-ang Chung Wei-Hung Liu 劉威宏 |
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Wei-Hung Liu 劉威宏 |
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Wei-Hung Liu 劉威宏 none |
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Wei-Hung Liu |
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2012 |
url |
http://ndltd.ncl.edu.tw/handle/37264848055131527200 |
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