Summary: | 碩士 === 國立嘉義大學 === 水生生物科學系研究所 === 100 === The Lernaeopodidae is a family of parasitic copepods found mainly on marine fishes. It is the second largest family of the order Siphonostomatoida, consisting of over 250 species in 48 genera. Only the female lernaeopodids are parasitic. They attach to fish host by a pair of modified maxillae or with a characteristic organ called bulla, which is held by the maxillae and inserted into the skin on various parts of the fish including both teleosts and chondrichthyes. The male is dwarf, attaching to the body of the female, and usually dies soon after mating. This report, deals with 1217 females and 359 males of lernaeopodids taken from 23 species of marine fishes of Taiwan collected between 1997 and 2011. The were identified belonging to 11 species in 9 genera. All of them are new to Taiwan. They are : Parabrachiella trichiuri (Yamaguti, 1939) and P. albida (Rangnekar, 1956); Thysanote chalermwati Piasecki, et al, 2008 and T. triloba Pearse, 1952; Charopinopsis equternia ( Wilson, 1936 ); Brachiella thynni Cuvier, 1817; Ommatokoita elongata (Grant, 1827); Pseudocharopinus kabatai Pillai, 1967; Clavellotis branchiostegi (Yamaguti, 1939); Cryptova limbifera Kabata, 1992 and Naobranchia auriculata Shiino, 1958. In this study, we amplified some particular segments of 18S ribosomal RNA gene and Mitochondrial DNA cytochrome oxidase subunit I were amplified with polymerase chain reaction ( PCR ) in order to obtain DNA sequences. Altogether 11 sequences were obtained from these lernaeopodids. The 18S rRNA was found to be useul in reconstruction of phylogenetic tree of genera. Besides, mtCOI were found to be helpful in identifying the species of lernaeopodids.
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