Cell Capture Microfluidic Device Integrated with Laser-induced Heating for Local Denaturation of DNA Fibers from a Cell Treated with Drugs

• Main Author: 黃一倉
• Other Authors: 洪敏勝
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/50012228281940253863

碩士 === 國立嘉義大學 === 生物機電工程學系研究所 === 100 === This study combines a microfluidic device and laser-induced heating system to develop a single cell capture that has been treated with drugs and partial cellular DNA denaturation microfluidic system. The microfluidic device utilized in this study consists of PDMS. The flow channel design consists mainly of cell capture and release, and DNA stretching and analysis zone. The concept of single cell capture is use the flow rate different between main flow channel and branch channel. The laser is focused on the laser heating zone to generate a bubble to release the captured cell. Finally, DNA stretching and partial cellular DNA denaturation is demonstrated in DNA stretching and analysis zone after cell lysis. The results show that larger hydrodynamic stresses act on the cell and move cell to flow through the micro-structure at high flow rate of 1.2×10^4μm/s, reducing the efficiency of cell capture. At low flow rate of 80μm/s, slight hydrodynamic effect on the cell and the cell can be captured; the capture efficiency is thus increased. In addition, when velocity in main flow channel is less than 80μm/s and laser power is 164mW, the cell can be released by expanded bubble after laser irradiation. The released cell flows into DNA analysis zone and is captured by micro-structure. The cell lysis and DNA extending are achieved after 1% SDS injection. Finally, the laser is used to irradiate the DNA fibers anchored between micro-pillars. When the laser power is 47mW, 30 seconds of irradiation, the partial DNA denaturation can be observed.