Suppression of the IRF-3 signaling pathway by the sfRNA of Japanese encephalitis virus is TANK-binding kinase-1 independent
碩士 === 國立東華大學 === 生命科學系 === 100 === Noncoding RNA (ncRNA) plays a critical role in modulating various stages of viral life cycle. Abundant ncRNA (named sfRNA), representing a highly conserved region of the 3’-untranslated region (UTR) of Japanese encephalitis virus (JEV), accumulated in JEV-infected...
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ndltd-TW-100NDHU51080092018-04-29T04:16:33Z http://ndltd.ncl.edu.tw/handle/5y5ja9 Suppression of the IRF-3 signaling pathway by the sfRNA of Japanese encephalitis virus is TANK-binding kinase-1 independent 日本腦炎病毒sfRNA抑制IRF-3訊息傳遞路徑不需TBK1蛋白質 Shu-Fan Liou 劉書帆 碩士 國立東華大學 生命科學系 100 Noncoding RNA (ncRNA) plays a critical role in modulating various stages of viral life cycle. Abundant ncRNA (named sfRNA), representing a highly conserved region of the 3’-untranslated region (UTR) of Japanese encephalitis virus (JEV), accumulated in JEV-infected cells. Previous work in our laboratory demonstrated that transfecting sfRNA into JEV-infected cells inhibited phosphorylation of interferon regulatory factor-3 (IRF-3), blocked roughly 30% of IRF-3 nuclear translocation, and reduced interferon-β by 50%. In this study, transfection of sfRNA had no effect on TANK-binding kinase 1 (TBK1) protein expression or its phosphorylation. Furthermore, the sfRNA binds to viral NS5 protein, but not to the IRF-3 or RIG-I molecules as demonstrated by ribonucleoportein co-immunoprecipitated (RIP) assay. RNAi knockdown of TBK1 resulted in enhancement of IRF-3 phosphorylation and reduction of virus particles. Taken together, these results indicated that suppression of the IRF-3 signaling pathway by the sfRNA is TBK1 independent. JEV induced anti-viral responses may be different from other viruses. Further research is needed to better understand the detailed evasion mechanisms mediated by the sfRNA in host innate immune responses. Ruey-Yi Chang 張瑞宜 2012 學位論文 ; thesis 57 |
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碩士 === 國立東華大學 === 生命科學系 === 100 === Noncoding RNA (ncRNA) plays a critical role in modulating various stages of viral life cycle. Abundant ncRNA (named sfRNA), representing a highly conserved region of the 3’-untranslated region (UTR) of Japanese encephalitis virus (JEV), accumulated in JEV-infected cells. Previous work in our laboratory demonstrated that transfecting sfRNA into JEV-infected cells inhibited phosphorylation of interferon regulatory factor-3 (IRF-3), blocked roughly 30% of IRF-3 nuclear translocation, and reduced interferon-β by 50%. In this study, transfection of sfRNA had no effect on TANK-binding kinase 1 (TBK1) protein expression or its phosphorylation. Furthermore, the sfRNA binds to viral NS5 protein, but not to the IRF-3 or RIG-I molecules as demonstrated by ribonucleoportein co-immunoprecipitated (RIP) assay. RNAi knockdown of TBK1 resulted in enhancement of IRF-3 phosphorylation and reduction of virus particles. Taken together, these results indicated that suppression of the IRF-3 signaling pathway by the sfRNA is TBK1 independent. JEV induced anti-viral responses may be different from other viruses. Further research is needed to better understand the detailed evasion mechanisms mediated by the sfRNA in host innate immune responses.
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Ruey-Yi Chang |
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Ruey-Yi Chang Shu-Fan Liou 劉書帆 |
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Shu-Fan Liou 劉書帆 |
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Shu-Fan Liou 劉書帆 Suppression of the IRF-3 signaling pathway by the sfRNA of Japanese encephalitis virus is TANK-binding kinase-1 independent |
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Shu-Fan Liou |
title |
Suppression of the IRF-3 signaling pathway by the sfRNA of Japanese encephalitis virus is TANK-binding kinase-1 independent |
title_short |
Suppression of the IRF-3 signaling pathway by the sfRNA of Japanese encephalitis virus is TANK-binding kinase-1 independent |
title_full |
Suppression of the IRF-3 signaling pathway by the sfRNA of Japanese encephalitis virus is TANK-binding kinase-1 independent |
title_fullStr |
Suppression of the IRF-3 signaling pathway by the sfRNA of Japanese encephalitis virus is TANK-binding kinase-1 independent |
title_full_unstemmed |
Suppression of the IRF-3 signaling pathway by the sfRNA of Japanese encephalitis virus is TANK-binding kinase-1 independent |
title_sort |
suppression of the irf-3 signaling pathway by the sfrna of japanese encephalitis virus is tank-binding kinase-1 independent |
publishDate |
2012 |
url |
http://ndltd.ncl.edu.tw/handle/5y5ja9 |
work_keys_str_mv |
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