Studies on the production of prodigiosin by marine Vibrio sp. strain C1-TDSG02-1 isolated from marine sediments

• Main Authors: Jun-Qi Wu, 吳俊錡
• Other Authors: Jimmy Kuo
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/85799391902071959669

碩士 === 國立東華大學 === 海洋生物科技研究所 === 100 === The marine ecosystem provides a unique ecological environment for diverse microorganisms with the potential to produce secondary metabolites with biological activities. A marine-derived, gram-negative γ-proteobacteria, Vibrio sp. C1-TDSG02-1, was isolated from Siaogang Harbor, Taitung, eastern Taiwan for its ability to produce bioactive metabolites. This red-pigmented antibiotic was identified as prodigiosin, which has anti-bacterial, anti-fungal and anti-tumor activities. To increase the production of prodigiosin, the growth of C1-TDSG02-1 was studied under different growth media and cultivation conditions. Bacterial growth is measured by absorbance at 600 nm. A paper-disc agar-diffusion assay was used to quantitatively measure the antimicrobial activity of the antibiotic agent against indictor bacterium Staphylococcus aureus. The concentration of prodigiosin was determined spectrophotometrically by measuring the absorbance value at 535 nm. Among the media tested, medium with nutrient broth supplemented with 1% starch and 0.5% glycerol as carbon source, as well as 1% glycine, and 0.2% tryptone as nitrogen source, favored the production of prodigiosin at 30°C in shake flask fermentation. The yield of prodigiosin in the optimal medium was 483 mg/L for fed-batch fermentation after four days of cultivation in a 10-L stirred tank fermentor, which was 1.12 times higher than by shake flask, with highest prodigiosin production after three days of cultivation.