| Summary: | 碩士 === 國立宜蘭大學 === 生物技術研究所碩士班 === 100 === Cordyceps militaris is a rare and exotic Chinese medicinal mushroom. This traditional
medicine has long been used by Chinese for thousands of years. Its chemical composition and
pharmacological effects were similar to Cordyceps sinensis. Secondary metabolites of
biologically active compounds – cordycepin (3’-deoxyadenosine). In recent years, cordycepin
has many pharmacological effects, such as immune regulation, anti-cancer, anti-inflammation,
and anti-virus.The objectives of this study were tried extract cordycepin from Cordyceps
militaris solid fermentative, and determine the possible anti-inflammation mechanism,
porcine reproductive and respiratory syndrome virus and secondary bacterial infection
whether the inhibition of pro-inflammatory cytokine mRNA expression with that of PRRSV
replication by hot water extract from Cordyceps militaris (CMHW) in porcine alveolar
macrophage (PAM). The results demonstrate that CMHW inhibit COX-2 expression, which
will reduce the downstream expression of NO. In addition, CMHW could inhibit the MAPK
and IKK casades via suppress the phosphorylation extent in JNK, p38 and IкB ultimately
inactivate the transcription factor such as activation protein 1 (AP-1) and nuclear factor кB
(NF-кB), and further inhibition of pro-inflammatory cytokines (TNF-α、IL-1β、IL-6). In
simulation experiments, PRRSV infection or secondary bacterial infection, CMHW can
significantly reduce TNF-α、IL-1β mRNA expression and the replication of PRRSV. The
CMHW exert high potential in feed additives development which could applied in swine
inflammatory related diseases caused by PRRSV and bacteria infection, and blocking the
replication of PRRSV. Reduce the economic losses of pig industry.
|
|---|
