Studies on anti-inflammatory and anti-PRRSV by Cordyceps militaris hot water

• Main Authors: Wang, Szukai, 王思凱
• Other Authors: Cheng, Yeonghsiang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/33181918253523033384

碩士 === 國立宜蘭大學 === 生物技術研究所碩士班 === 100 === Cordyceps militaris is a rare and exotic Chinese medicinal mushroom. This traditional medicine has long been used by Chinese for thousands of years. Its chemical composition and pharmacological effects were similar to Cordyceps sinensis. Secondary metabolites of biologically active compounds – cordycepin (3’-deoxyadenosine). In recent years, cordycepin has many pharmacological effects, such as immune regulation, anti-cancer, anti-inflammation, and anti-virus.The objectives of this study were tried extract cordycepin from Cordyceps militaris solid fermentative, and determine the possible anti-inflammation mechanism, porcine reproductive and respiratory syndrome virus and secondary bacterial infection whether the inhibition of pro-inflammatory cytokine mRNA expression with that of PRRSV replication by hot water extract from Cordyceps militaris (CMHW) in porcine alveolar macrophage (PAM). The results demonstrate that CMHW inhibit COX-2 expression, which will reduce the downstream expression of NO. In addition, CMHW could inhibit the MAPK and IKK casades via suppress the phosphorylation extent in JNK, p38 and IкB ultimately inactivate the transcription factor such as activation protein 1 (AP-1) and nuclear factor кB (NF-кB), and further inhibition of pro-inflammatory cytokines (TNF-α、IL-1β、IL-6). In simulation experiments, PRRSV infection or secondary bacterial infection, CMHW can significantly reduce TNF-α、IL-1β mRNA expression and the replication of PRRSV. The CMHW exert high potential in feed additives development which could applied in swine inflammatory related diseases caused by PRRSV and bacteria infection, and blocking the replication of PRRSV. Reduce the economic losses of pig industry.