| Summary: | 碩士 === 國立宜蘭大學 === 生物資源學院碩士在職專班 === 100 === Eucalyptus is an important pulpwood genus distribution widely and was introduced to Taiwan over a century ago. The reported bio-activities of the leaves of eucalypts were antioxidative and antimicrobial (including bacteria, mildews and wood decaying fungi). In this study, essential oils from the leaves of 4 species, i.e., E. urophylla, E. grandis, E. camaldulensis, and E. citriodora were extracted using water distillation, and were evaluated for their anti-inflammatory activity in murine macrophages cell lines RAW 264.7. Experimental results showed that essential oils of E. citriodora had the best efficacy in NO inhibition. Essential oils of E. citriodora were fractioned into A~H fractions using column chromatography. We found that Fraction F inhibited TNF-alpha, IL-6, NO, iNOS and COX-2 expression in LPS-stimulated macrophages. Furthermore, the phosphorylation of ERK1/2, p38, PKC-alpha, PKC-epsilon and PKC-delta induced by LPS were inhibited by Fraction F, while the phosphorylation of JNK1/2 was not reduced by Fraction F. Fraction F also inhibited NF-kappaB activation in LPS-stimulated macrophages. Our current results indicated that Fraction F is the major anti-inflammatory ingredient of E. citriodora.
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