Feasibility of Using Complex Bioremediation Technologies to Remove Diesel in the Soil

• Main Authors: Hung-Hau Huang, 黃泓豪
• Other Authors: Jiunn-Jyi Lay
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/18478487771567992655

碩士 === 國立高雄第一科技大學 === 環境與安全衛生工程研究所 === 100 === The purpose of this study was used a complex bioremediation technology to decompose the diesel of soil. For fulfilling this study, a simulated diesel polluted soil was performed for the bioaugmentation, rhizoremediation and complex bioremediation technologies. The biological agent for bioaugmentation and complex bioremediation technologies was a liquid obtained from an anaerobic hydrogen-producing fermenter. This agent contained lots of microorganisms including Bacillus sp. and Clostridium sp., nutrients, and organics. Moreover, Bermudagrass was conducted to rhizoremediation and complex bioremediation technologies. Experimental results of bioaugmentation demonstrated that the only 1.7% of diesel was decomposed. Initially, a high carbon dioxide producing rate (1.92 mL/day) was obtained at 100 mL vial bioactivity experiments. The observation clarified that the agent without acclimation with diesel was hard to decompose diesel but it still had bioactivity. The rhizoremediation experimental results indicated that the diesel concentration would not directly affect the Bermudagrass germination. However, the concentration will significantly affect the growth of Bermudagrass. Not surprisingly, only 0.5% diesel decomposing rate was obtained. The complex technology in this study was combined bioaugmentation and rhizoremediation. That is to say, the agent was watered on the soil with high density of Bermudagrass (38 plant/cm2). In first seven days, 76 ± 3% of diesel was decomposed. After forty two days, more than 80% decomposing rate was occurred at all 10 samples which conditions was varied at concentration of the agent and diesel. The results were obvious that during the period of Bermudagrass germination with the agent had a high potential to decompose diesel.