Development of DNA vaccine against waterfowl parvovirus

• Main Authors: Jin-Jie Lin, 林金潔
• Other Authors: Chun-Yen Chu
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/27591797606632232242

碩士 === 國立屏東科技大學 === 動物疫苗科技研究所 === 100 === Waterfowl parvovirus is a highly contagious infection which causes dreadful enteritis and high mortality in ducklings and geese, growth retardation of adult birds and economic losses. At present, the commercial traditional inactivated or attenuated virus vaccine is used to prevent infection, but whole virus production is limited. In previous study, DNA vaccine can improve the defect of traditional vaccine, after the plasmid DNA is captured by the antigen presenting cells, expressed protein antigen can stimulate both humoral and cellular immune response. It have characteristics of long-term immune memory, easy preparation, low cost, easy to transport and storage. In addition, unmethylated CpG oligodeoxynucleotides (CpG ODNs) could enhance immunity, induced antibody response, and IFN-γ mRNA expression of PBMC is significantly increased in CpG ODN treated ducklings compared to control group. The objective of our study is to manufacture large scale of plasmid DNA, and combine with CpG2-P3 ODN adjuvant or CpG2-P4 ODN adjuvant to immunize ducks. The results showed that priming with a DNA vaccine and boosting with the VP2 recombinant protein with designed CpG2-P3 adjuvant or CpG2-P4 adjuvant could induce specific antibodies titer, cell proliferation, percentage of CD4+ and CD8+ cells in PBMC was significantly (p<0.05) higher than other groups. In addition, the relative fold induction of mRNA expression of IFN-α, IFN-γ, IL-6 and IL-12 in PBMC isolated from ducks was significantly (p<0.05) upregulated on D14 and D28 in comparison other groups. In conclusion, priming with a DNA vaccine and boosting with the VP2 recombinant protein with CpG2-P3 adjuvant or CpG2-P4 adjuvant is able to improve the efficacy of DNA vaccines and provide an effective approach to protect ducklings from waterfowl parvovirus.