Functional study of aquaporin 8aa in skin of zebrafish larva
碩士 === 國立臺灣師範大學 === 生命科學研究所 === 100 === Aquaporins (AQPs) are integral membrane proteins that facilitate water transport across cell membrane. In addition, some type of AQPs was also found to facilitate diffusion of CO2, glycerol, NH3, and urea. Recently, in vitro studies with Xenopus oocytes showed...
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ndltd-TW-100NTNU51120302016-03-28T04:20:21Z http://ndltd.ncl.edu.tw/handle/79538528963802185465 Functional study of aquaporin 8aa in skin of zebrafish larva 水通道蛋白8aa在斑馬魚仔魚上的功能性研究 Kao, Yang-Yen 高揚彥 碩士 國立臺灣師範大學 生命科學研究所 100 Aquaporins (AQPs) are integral membrane proteins that facilitate water transport across cell membrane. In addition, some type of AQPs was also found to facilitate diffusion of CO2, glycerol, NH3, and urea. Recently, in vitro studies with Xenopus oocytes showed that AQP8 is able to facilitate CO2/NH3 diffusion. However, in vivo study was lacking to support AQP’s function in CO2/NH3 transport. In this study, AQP8AA were identified in the gills and larval skin of zebrafish. Triple in situ hybridization / immunohistochemistry localized AQP8AA in two subtypes of ionocytes (HR cells and NaR cells). The protein and mRNA expressions of AQP8aa were induced by high-ammonia acclimation (10 mM NH4+) but not hypercapnia water acclimation (1% CO2). Using morpholino oligonucleotides to knockdown AQP8AA protein expression and a scanning ion-selective technique (SIET) to analyze carbonic acid (H+) and NH4+ transport at the skin ionocytes of larvae, this study suggests that AQP8AA facilitates CO2/NH3 transport in the skin ionocytes. Lin, Li-Yih 林豊益 2011 學位論文 ; thesis 59 zh-TW |
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碩士 === 國立臺灣師範大學 === 生命科學研究所 === 100 === Aquaporins (AQPs) are integral membrane proteins that facilitate water transport across cell membrane. In addition, some type of AQPs was also found to facilitate diffusion of CO2, glycerol, NH3, and urea. Recently, in vitro studies with Xenopus oocytes showed that AQP8 is able to facilitate CO2/NH3 diffusion. However, in vivo study was lacking to support AQP’s function in CO2/NH3 transport. In this study, AQP8AA were identified in the gills and larval skin of zebrafish. Triple in situ hybridization / immunohistochemistry localized AQP8AA in two subtypes of ionocytes (HR cells and NaR cells). The protein and mRNA expressions of AQP8aa were induced by high-ammonia acclimation (10 mM NH4+) but not hypercapnia water acclimation (1% CO2). Using morpholino oligonucleotides to knockdown AQP8AA protein expression and a scanning ion-selective technique (SIET) to analyze carbonic acid (H+) and NH4+ transport at the skin ionocytes of larvae, this study suggests that AQP8AA facilitates CO2/NH3 transport in the skin ionocytes.
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author2 |
Lin, Li-Yih |
author_facet |
Lin, Li-Yih Kao, Yang-Yen 高揚彥 |
author |
Kao, Yang-Yen 高揚彥 |
spellingShingle |
Kao, Yang-Yen 高揚彥 Functional study of aquaporin 8aa in skin of zebrafish larva |
author_sort |
Kao, Yang-Yen |
title |
Functional study of aquaporin 8aa in skin of zebrafish larva |
title_short |
Functional study of aquaporin 8aa in skin of zebrafish larva |
title_full |
Functional study of aquaporin 8aa in skin of zebrafish larva |
title_fullStr |
Functional study of aquaporin 8aa in skin of zebrafish larva |
title_full_unstemmed |
Functional study of aquaporin 8aa in skin of zebrafish larva |
title_sort |
functional study of aquaporin 8aa in skin of zebrafish larva |
publishDate |
2011 |
url |
http://ndltd.ncl.edu.tw/handle/79538528963802185465 |
work_keys_str_mv |
AT kaoyangyen functionalstudyofaquaporin8aainskinofzebrafishlarva AT gāoyángyàn functionalstudyofaquaporin8aainskinofzebrafishlarva AT kaoyangyen shuǐtōngdàodànbái8aazàibānmǎyúzǐyúshàngdegōngnéngxìngyánjiū AT gāoyángyàn shuǐtōngdàodànbái8aazàibānmǎyúzǐyúshàngdegōngnéngxìngyánjiū |
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1718212918426730496 |