Antihyperglycemic Effect of Hydrolysate of Chlorella and Freshwater Clam

• Main Authors: Pi-Yu Liu, 劉碧玉
• Other Authors: Jenn-Shou Tsai
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/29311886720431019642

碩士 === 國立臺灣海洋大學 === 食品科學系 === 100 === pulverized chlorella and freshwater clam were used as new material in this study. Pulverized chlorella were hydrolyzed by cellulase and protease at 50℃ for 3 hours. The freshwater clam muscle was extracted using hot water. The residual meat was first freeze-dried, then hydrolyzed at 50oC by protease to obtain freshwater clam hydrolysate. The inhibitory effect of Chlorella (CH) and freshwater clam hydrolysates (PX) on activity of α-glucosidase and DPP4 (dipepidy peptidase 4) were investigated, and the physiological effect on regulation of blood sugar was also evaluated. Pulverized chlorella hydrolysate was separated into five fractions by size exclusion chromatography on a Sephadex G-25 gel with BPG column. The fraction P2 (M.W.=860-740 Da) and P4 (M.W.=350-300 Da) of hydrolysate showed the higher inhibition on α-glucosidase (AG) with IC50 being 1.42 and 3.62 mg/ml, respectively. The fraction P4 was further analyzed by RP-HPLC with HILIC column, the peak H2 had the better inhibitory activity againt AG. Freshwater clam hydrosate was filtered with membrane cut-off at 1000 Da. The IC50 of filtered (M.W. < 1000 Da) against AG and DPP4 were 25.69 and 8.54 mg/ml, respectively. After 8 weeks of feeding the type 2 diabetic rats induced by STZ with CH/PX reduced 11.6 and 10.5% plasma sugar at the 60 and 120 minutes in glucose tolerance test. Diabetic rats fed CH, PX and CH/PX siginifcantly decreased plasma glucose by 8.1, 3.7 and 9.6%, plasma total cholesterol (24.2, 37.3 and 29.1%, respectively) and triglyceride (5.3, 12.6 and 8.8%, respectively) in fasting. Plasma insulin and HOMA-IR (homeostatic model assessment insulin resistance ) levels decreased by 38.0 and 51.95%, respectively, in diabetic rats fed with CH/PX.