Detection of steroid hormones in saliva with surface-assisted laser desorption/ionization mass spectrometry

Detection of steroid hormones in saliva with surface-assisted laser desorption/ionization mass spectrometry

碩士 === 國立臺東大學 === 生命科學系碩士班 === 100 === In our study, we developed a steroid hormones analysis with surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) method. The Au nanoparticles (AuNPs) were used as SALDI matrices. We tested four steroid hormones: cortisone, hydrocortisone, p...

Full description

Bibliographic Details
Main Authors: Ching-Hui Chen, 陳靜慧
Other Authors: Cho-Chun Hu, Pai-Lu Wei
Format: Others
Language:zh-TW
Published: 2012
Online Access:http://ndltd.ncl.edu.tw/handle/pg9383
id ndltd-TW-100NTTU5105009
record_format oai_dc
spelling ndltd-TW-100NTTU51050092019-09-03T03:43:14Z http://ndltd.ncl.edu.tw/handle/pg9383 Detection of steroid hormones in saliva with surface-assisted laser desorption/ionization mass spectrometry 以表面輔助雷射脫附游離法偵測唾液中類固醇荷爾蒙 Ching-Hui Chen 陳靜慧 碩士 國立臺東大學 生命科學系碩士班 100 In our study, we developed a steroid hormones analysis with surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) method. The Au nanoparticles (AuNPs) were used as SALDI matrices. We tested four steroid hormones: cortisone, hydrocortisone, progesterone, and testosterone. The effects of the concentrations of AuNPs, the concentrations of ammonium citrate buffer, pH of ammonium citrate buffer, and laser fluence on the signal intensity were investigated. The optimum conditions were contained 45 nM AuNPs as matrices prepared in 0.096 mM ammonium citrate (pH 5.0). The laser fluence was 20.62 Cho-Chun Hu, Pai-Lu Wei 胡焯淳、魏百祿 2012 學位論文 ; thesis 60 zh-TW
collection NDLTD
language zh-TW
format Others
sources NDLTD
description 碩士 === 國立臺東大學 === 生命科學系碩士班 === 100 === In our study, we developed a steroid hormones analysis with surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) method. The Au nanoparticles (AuNPs) were used as SALDI matrices. We tested four steroid hormones: cortisone, hydrocortisone, progesterone, and testosterone. The effects of the concentrations of AuNPs, the concentrations of ammonium citrate buffer, pH of ammonium citrate buffer, and laser fluence on the signal intensity were investigated. The optimum conditions were contained 45 nM AuNPs as matrices prepared in 0.096 mM ammonium citrate (pH 5.0). The laser fluence was 20.62
author2 Cho-Chun Hu, Pai-Lu Wei
author_facet Cho-Chun Hu, Pai-Lu Wei
Ching-Hui Chen
陳靜慧
author Ching-Hui Chen
陳靜慧
spellingShingle Ching-Hui Chen
陳靜慧
Detection of steroid hormones in saliva with surface-assisted laser desorption/ionization mass spectrometry
author_sort Ching-Hui Chen
title Detection of steroid hormones in saliva with surface-assisted laser desorption/ionization mass spectrometry
title_short Detection of steroid hormones in saliva with surface-assisted laser desorption/ionization mass spectrometry
title_full Detection of steroid hormones in saliva with surface-assisted laser desorption/ionization mass spectrometry
title_fullStr Detection of steroid hormones in saliva with surface-assisted laser desorption/ionization mass spectrometry
title_full_unstemmed Detection of steroid hormones in saliva with surface-assisted laser desorption/ionization mass spectrometry
title_sort detection of steroid hormones in saliva with surface-assisted laser desorption/ionization mass spectrometry
publishDate 2012
url http://ndltd.ncl.edu.tw/handle/pg9383
work_keys_str_mv AT chinghuichen detectionofsteroidhormonesinsalivawithsurfaceassistedlaserdesorptionionizationmassspectrometry
AT chénjìnghuì detectionofsteroidhormonesinsalivawithsurfaceassistedlaserdesorptionionizationmassspectrometry
AT chinghuichen yǐbiǎomiànfǔzhùléishètuōfùyóulífǎzhēncètuòyèzhōnglèigùchúnhéěrméng
AT chénjìnghuì yǐbiǎomiànfǔzhùléishètuōfùyóulífǎzhēncètuòyèzhōnglèigùchúnhéěrméng
_version_ 1719242395057389568

Similar Items