Effects of imidacloprid in sub-lethal dosage to honey bee, Apis mellifera

• Main Authors: I-Tse Lu, 呂易澤
• Other Authors: 楊恩誠
• Format: Others
• Language: en_US
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/66259490369748331015

碩士 === 國立臺灣大學 === 昆蟲學研究所 === 100 === Imidacloprid has been proved that it makes harmful influence on insect, and there is a great deal of research on honey bee. While the effect of imidacloprid on honey bee has been extensively investigated, the impact of sub-lethal dosage on colony health is relatively important, especially on chronic influence. This thesis studies the transference of imidacloprid from fields to colony. The research focuses on the analysis of residual imidacloprid concentration in honey bee hive including honey, larvae, and eclosion adults after exposing in poisoned environment. Firstly, the colonies were fed with imidacloprid syrup in range of 5-100 μg/L directly in hive, and then high performance liquid chromatography-diode array detector (HPLC-DAD) was used to detect the residual imidacloprid concentration. Moreover, the olfactory learning ability of eclosion adults is examined by proboscis extension reflex (PER). The findings suggest that the residual imidacloprid was only detected in honey samples after the colonies fed with imidacloprid syrup 100 μg/L, and the concentration reached 35.45 μg/L. The results of learning ability examine showed that there were no significant differences between normal honey bees and treated honey bees which were fed with inidacloprid syrup in range of 25-100 μg/L. These findings indicate that imidacloprid could not make significant impact on colony health through honey transference. However, some studies found the pollen had high residual imidacloprid in beehive, but the influence it caused is still unclear. Therefore, it needs more investigations to explore the possible transferred pathway of imidacloprid and whether the accumulated imidacloprid concentration in beehive makes chronic impact on colony health in the future.