Multiphoton fluorescence lifetime imaging microscopy (FLIM) of Bilirubin oxidants of oral cancer cells
碩士 === 國立臺灣大學 === 醫學工程學研究所 === 100 === We have observed the red fluorescence of five oral cell lines with multi-photon fluorescence lifetime and intensity. With lifetime trace, single photon, and two photon fluorescence spectrum, we found out that biliverdin is one of red fluorescence source (exciti...
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ndltd-TW-100NTU055300172015-10-13T21:45:44Z http://ndltd.ncl.edu.tw/handle/15961550471240664525 Multiphoton fluorescence lifetime imaging microscopy (FLIM) of Bilirubin oxidants of oral cancer cells 以多光子螢光生命週期影像顯微術來鑑別正常與口腔癌細胞 Cheng-Jie Huang 黃成杰 碩士 國立臺灣大學 醫學工程學研究所 100 We have observed the red fluorescence of five oral cell lines with multi-photon fluorescence lifetime and intensity. With lifetime trace, single photon, and two photon fluorescence spectrum, we found out that biliverdin is one of red fluorescence source (exciting spectrum peak around ~660nm). Four oral cancer cells from tongue and a non-cancer oral cell lines: S-G (normal oral cell as control group), DOK (pre-cancer oral cell), OECM-1, SCC-15, and HSC-3(different stage of malignancy) were investigated by a femtosecond Cr:forsterite laser -based multi-photon fluorescence lifetime image. Due to shift of metabolic status, we used two component to fit lifetime histgrom and detected that DOK and OECM-1 has longer t1 lifetime(~500ps), SCC-15 and HSC-3 have relatively short 370ps, and normal oral, S-G cells have much shorter t1 lifetime(~270ps). In addition, S-G cell have visible shorter t2 lifetime (~1.6ns), cancer cells general longer (~1.9ns). With our study result, we believe this lifetime of biliverdin fluorescence is potential to serve as an diagnostic index on the stage and malignancy of oral cancers in the future. Tzu-Ming Liu 劉子銘 2012 學位論文 ; thesis 58 zh-TW |
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碩士 === 國立臺灣大學 === 醫學工程學研究所 === 100 === We have observed the red fluorescence of five oral cell lines with multi-photon fluorescence lifetime and intensity. With lifetime trace, single photon, and two photon fluorescence spectrum, we found out that biliverdin is one of red fluorescence source (exciting spectrum peak around ~660nm). Four oral cancer cells from tongue and a non-cancer oral cell lines: S-G (normal oral cell as control group), DOK (pre-cancer oral cell), OECM-1, SCC-15, and HSC-3(different stage of malignancy) were investigated by a femtosecond Cr:forsterite laser -based multi-photon fluorescence lifetime image. Due to shift of metabolic status, we used two component to fit lifetime histgrom and detected that DOK and OECM-1 has longer t1 lifetime(~500ps), SCC-15 and HSC-3 have relatively short 370ps, and normal oral, S-G cells have much shorter t1 lifetime(~270ps). In addition, S-G cell have visible shorter t2 lifetime (~1.6ns), cancer cells general longer (~1.9ns). With our study result, we believe this lifetime of biliverdin fluorescence is potential to serve as an diagnostic index on the stage and malignancy of oral cancers in the future.
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author2 |
Tzu-Ming Liu |
author_facet |
Tzu-Ming Liu Cheng-Jie Huang 黃成杰 |
author |
Cheng-Jie Huang 黃成杰 |
spellingShingle |
Cheng-Jie Huang 黃成杰 Multiphoton fluorescence lifetime imaging microscopy (FLIM) of Bilirubin oxidants of oral cancer cells |
author_sort |
Cheng-Jie Huang |
title |
Multiphoton fluorescence lifetime imaging microscopy (FLIM) of Bilirubin oxidants of oral cancer cells |
title_short |
Multiphoton fluorescence lifetime imaging microscopy (FLIM) of Bilirubin oxidants of oral cancer cells |
title_full |
Multiphoton fluorescence lifetime imaging microscopy (FLIM) of Bilirubin oxidants of oral cancer cells |
title_fullStr |
Multiphoton fluorescence lifetime imaging microscopy (FLIM) of Bilirubin oxidants of oral cancer cells |
title_full_unstemmed |
Multiphoton fluorescence lifetime imaging microscopy (FLIM) of Bilirubin oxidants of oral cancer cells |
title_sort |
multiphoton fluorescence lifetime imaging microscopy (flim) of bilirubin oxidants of oral cancer cells |
publishDate |
2012 |
url |
http://ndltd.ncl.edu.tw/handle/15961550471240664525 |
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