Multiphoton fluorescence lifetime imaging microscopy (FLIM) of Bilirubin oxidants of oral cancer cells

• Main Authors: Cheng-Jie Huang, 黃成杰
• Other Authors: Tzu-Ming Liu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/15961550471240664525

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 100 === We have observed the red fluorescence of five oral cell lines with multi-photon fluorescence lifetime and intensity. With lifetime trace, single photon, and two photon fluorescence spectrum, we found out that biliverdin is one of red fluorescence source (exciting spectrum peak around ~660nm). Four oral cancer cells from tongue and a non-cancer oral cell lines: S-G (normal oral cell as control group), DOK (pre-cancer oral cell), OECM-1, SCC-15, and HSC-3(different stage of malignancy) were investigated by a femtosecond Cr:forsterite laser -based multi-photon fluorescence lifetime image. Due to shift of metabolic status, we used two component to fit lifetime histgrom and detected that DOK and OECM-1 has longer t1 lifetime(~500ps), SCC-15 and HSC-3 have relatively short 370ps, and normal oral, S-G cells have much shorter t1 lifetime(~270ps). In addition, S-G cell have visible shorter t2 lifetime (~1.6ns), cancer cells general longer (~1.9ns). With our study result, we believe this lifetime of biliverdin fluorescence is potential to serve as an diagnostic index on the stage and malignancy of oral cancers in the future.