The Study of Expression Patterns of 8 rrn Operons of Vibrio cholerae O139 in VBNC State
碩士 === 東吳大學 === 微生物學系 === 100 === “Viable but non-culturable” (VBNC) represents a state that cells are dormant and can not be cultured by standard growth media. The gram-negative bacteria can be induced to enter the VBNC state by starvation, cultured at low temperature, in high salinity, or in diffe...
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ndltd-TW-100SCU053810032015-10-13T21:12:27Z http://ndltd.ncl.edu.tw/handle/93245058470308846867 The Study of Expression Patterns of 8 rrn Operons of Vibrio cholerae O139 in VBNC State 霍亂弧菌 O139 進入活而不長狀態8 組 rrn 操縱組表現特性之研究 Hsiao-lan Wang 王筱嵐 碩士 東吳大學 微生物學系 100 “Viable but non-culturable” (VBNC) represents a state that cells are dormant and can not be cultured by standard growth media. The gram-negative bacteria can be induced to enter the VBNC state by starvation, cultured at low temperature, in high salinity, or in different pH. Many of human pathogenic bacteria can enter into the VBNC state, and retain their virulence during the state. This phenomena causes the blind spot in quarantine. In this paper, we investigate one of the human pathogens - the Vibrio cholera O139 which causing the cholera. In this study, we analyzed the differences among the cells in late log phase, in the progress of starvation-to-death, in the process of induction into VBNC state, and in BVNC state at RNA level by electrophoresis and Northern blot. Besides, specific expression primers were designed and RT-PCR was employed to analyse these 8 rrn operons expression patterns among the cells in late log phase, in the progress of starvation-to death, in the process of induction into VBCN state, and in VBNC state. According to the analysis result, we found that rrn a , rrn c, rrn f, rrn b and rrn b operons were constitutively expressed in the cells in late log phase, in the progress of starvation-to-death, in the process of induction into VBNC state, and in VBNC state. On the contrary, the rrn g operon was expressed in the late log phase and in starvation-to-death, but not in VBNC state. The expression pattern of rrn d, rrn e could not be verified in this study, because the lack of the proper specific expression patterns for these operons. none 路光予 2012 學位論文 ; thesis 116 zh-TW |
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碩士 === 東吳大學 === 微生物學系 === 100 === “Viable but non-culturable” (VBNC) represents a state that cells are dormant and can not be cultured by standard growth media. The gram-negative bacteria can be induced to enter the VBNC state by starvation, cultured at low temperature, in high salinity, or in different pH. Many of human pathogenic bacteria can enter into the VBNC state, and retain their virulence during the state. This phenomena causes the blind spot in quarantine. In this paper, we investigate one of the human pathogens - the Vibrio cholera O139 which causing the cholera. In this study, we analyzed the differences among the cells in late log phase, in the progress of starvation-to-death, in the process of induction into VBNC state, and in BVNC state at RNA level by electrophoresis and Northern blot. Besides, specific expression primers were designed and RT-PCR was employed to analyse these 8 rrn operons expression patterns among the cells in late log phase, in the progress of starvation-to death, in the process of induction into VBCN state, and in VBNC state. According to the analysis result, we found that rrn a , rrn c, rrn f, rrn b and rrn b operons were constitutively expressed in the cells in late log phase, in the progress of starvation-to-death, in the process of induction into VBNC state, and in VBNC state. On the contrary, the rrn g operon was expressed in the late log phase and in starvation-to-death, but not in VBNC state. The expression pattern of rrn d, rrn e could not be verified in this study, because the lack of the proper specific expression patterns for these operons.
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none Hsiao-lan Wang 王筱嵐 |
author |
Hsiao-lan Wang 王筱嵐 |
spellingShingle |
Hsiao-lan Wang 王筱嵐 The Study of Expression Patterns of 8 rrn Operons of Vibrio cholerae O139 in VBNC State |
author_sort |
Hsiao-lan Wang |
title |
The Study of Expression Patterns of 8 rrn Operons of Vibrio cholerae O139 in VBNC State |
title_short |
The Study of Expression Patterns of 8 rrn Operons of Vibrio cholerae O139 in VBNC State |
title_full |
The Study of Expression Patterns of 8 rrn Operons of Vibrio cholerae O139 in VBNC State |
title_fullStr |
The Study of Expression Patterns of 8 rrn Operons of Vibrio cholerae O139 in VBNC State |
title_full_unstemmed |
The Study of Expression Patterns of 8 rrn Operons of Vibrio cholerae O139 in VBNC State |
title_sort |
study of expression patterns of 8 rrn operons of vibrio cholerae o139 in vbnc state |
publishDate |
2012 |
url |
http://ndltd.ncl.edu.tw/handle/93245058470308846867 |
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