High Density Culture of Chlorella sp.

• Main Authors: Chi, Wei-Yu, 紀威宇
• Other Authors: 李順來
• Format: Others
• Language: zh-TW
• Published: 101
• Online Access: http://ndltd.ncl.edu.tw/handle/86715848065498943198

碩士 === 南台科技大學 === 生物科技系 === 100 === Abstract The purpose of this study is to study various carbon sources, including sodium carbonate, calcium carbonate and fixed carbon dioxide solution, to optimize the process for chlorella sp. product. The spectrophotometric measurement of the absorbance of optical density (optical density, OD) of culture broth is used to obtain the concentration of algae and the dry weight of the algae The experimental results show that the dry weight of chlorella achieving 1.7g/L dry weight. We addition 2.5g/L sodium carbonate, whilst the dry weight of chlorella reaching 1.42g/L as using calcium carbonate as carbon source at the same concentration. In the thesis, fixation of carbon dioxide by sodium hydroxide is studied to obtain enriched carbon dioxide solution. The cultivation of chlorella sp. in above solution is processed. The result shows that 1.57g/L of chlorella dry cells is obtained. The thesis demonstrates that addition of sodium carbonate, calcium carbonate, or enriched carbon dioxide solution very be a promising strategy to enhance the growth of chlorella sp.