Exploring the roles of TCTP in central nervous system via GFAP-cre derived conditional knockout mice

• Main Authors: Shiou-shian Wu, 吳繡賢
• Other Authors: Sung-Ho Chen
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/46239609377418793714

碩士 === 慈濟大學 === 藥理暨毒理學碩士班/博士班 === 100 === Translation controlled tumor protein (TCTP) plays a major role in a broad array of biological processes. However, the TCTP-related biological process and interactive proteins still remain poorly characterized in central nervous system development. Our previous study showed that TCTP homozygous mutants (TCTP-/-) mice were embryonic lethality and the nestin-cre conditional knockout of TCTP caused perinatal lethality. The physiological role of TCTP underlying development and neurodegeneration of the brain were further evaluated by more specific deletion of TCTP in glial fibrillary acidic protein (GFAP) positive cells. The TCTPflox/flox mice crossed with GFAP-cre; TCTPflox/+ to generate tissue dependent conditional knockout mice of TCTP under the control of the GFAP promoter. We found that TCTP deficiency resulted in the decrease of body weight, cerebellum size and hair loss. Moreover, the TCTP cKO mice displayed dysfunction in motivation, and motor coordination and normal function in spatial memory, recognition memory, contextual learning and memory compared to the control littermates. The cerebellum of TCTP cKO mice were hypoplastic and impaired foliation and stunted purkinje cell dendrites. These findings suggest that TCTP play important roles in the development, differentiation and maturation of nervous system of the mice, especially in the development of cerebellum. Keyword: Astrocyte、conditional knockout、cerebellum development 、glial fibrillary acidic protein (GFAP)、Purkinje cell、 translationally controlled tumour protein (TCTP)