Discovery of a biomarker of oral cancer by MALDI-TOF MS analysis of protein profiles in oral cancer cell lines and cancer tissues

• Main Authors: Huang, Leiway, 黃笠崴
• Other Authors: Lung, Fengdi
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/19365771391786178597

碩士 === 東海大學 === 化學系 === 100 === Oral squamous cell carcinoma (OSCC) is a malignant and aggressive tumor which was occurred mostly in smokers, alcoholics, and betel squid chewers. OSCC is one of the top ten causes of death from cancers in Taiwan, therefore, there is an urgent need to develop a method for detecting oral cancer and identifying high risk individuals at the earlier stages. The goal of this study is to optimize experimental conditions for developing an efficient method for detecting the progression of oral cancer. We have extracted total proteins from six oral cancer cell lines, three normal cell lines and eight oral tumor tissues and their matched normal mucosas, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis of protein profiles. Cell lines and tissues were homogenized with protein extraction solution, after centrifugation, supernatant were desalted by solid phase extraction using C18 column. The desalted supernatant was analyzed by MALDI-TOF MS, and results demonstrated that the intensity of a protein fragment with molecular weight of 10.9 kD was correlated with progression of oral cancer. We have optimized experimental conditions and discovered a protein biomarker (10.9 kD) of oral cancer. This biomarker can be applied for the development of efficient methods for monitoring the progression of and diagnosis of oral cancer.