| Summary: | 碩士 === 國立臺北科技大學 === 生物科技研究所 === 100 === In this study, the aim is to develop a novel bone glue composed of tricalcium silicate and high proportion of type I collagen. The bone glue has plasticity in the early stage and will self-set after hydration to provide mechanical strength.
Tricalcium silicate (C3S) is one of the main components of Portland cement, with the ability of rapid hydration to harden in a short period of time. It was reported to induce the formation of bone-like apatite and with excellent bone binding capacity in vitro and in vivo. Type I collagen, the main distribution of the majority of connective tissue in the skin, hard bone and blood vessels, plays important roles in tissue remodeling, wound healing and bone reconstruction. Carboxymethyl cellulose (CMC) is a cellulose-based ring hydroxyl methyl substituted derivative. Depending on the degree of substitution, CMC provides different levels of the sticky property and has been widely used in biomedical products in the recent years.
In this experiment, sodium carboxymethyl cellulose with medium degree of substitution was applied to increase the adhesion property of the bone glue. Mechanical property test, surface morphology observation, crystalline phase analysis, in vitro pH value measurement, ions releasing, cytotoxicity test with MG 63 cell line and the drug releasing test including antibiotics and nonsteroidal anti-inflammatory were carried out.
The results indicated that bone glue containing 40% collagen, Glue 40, had the following advantages, including shorter working time and setting time, about 11 and 150 minutes, respectively; better adhesion ability in vitro; higher adhesive strength about 416 Kpa; higher tensile strength about 167 Kpa. After seven days curing in the simulated body fluid, Glue 40 was cemented to be block and the changes of pH value was controlled between 7~9. Furthermore, Glue 40 was selected for advanced investigations.
After seven days of curing in the saturated vapor of water, the compressive strength of Glue 40 increased with time and reached the maximum value of 500 Kpa. In addition, the additive of CMC not only enhanced the viscosity of the material, but also promoted the proliferation of MG 63 cells. Results of tetracycline releasing test showed that Glue 40 had the ability of initial burst, from 4% to 13%, and then maintained about 20%. Results of diclofenac sodium releasing test showed that after two days the release increased from 46% to 53%, and then maintained about 53%. Concluding the results of tests, Glue 40 has great potential to be a novel bone glue carrying with drugs.
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