| Summary: | 碩士 === 國立臺北科技大學 === 生物科技研究所 === 100 === The purpose of this research was to carry out the detailed in vitro study of guided bone tissue regeneration membrane. The fabricated membrane, P-C film, was made of type I collagen cross-linked using oligomeric proanthocyanidins (OPCs) as the cross-linking agent. The human osteoblast cell line (MG63) and human skin fibroblast cell line (WS1) were cultured for in vitro studies. The effects of P-C film on MG63 cell viability, adhesion, proliferation, alkaline phosphatase activity and differentiation were evaluated. In order to investigate the barrier ability of the tissue regeneration membrane, capillary flow porometer was used to analyze the pore size; separated co-culture experiments using MG63 and WS1 were carried out to observe the cell migration behavior.
The results showed that P-C film did not affect cell growth and had good biocompatibility. The cells attached to the P-C film surface increased with time. We also found that MG63 cells grew faster on P-C film, compared to the culture flask. On the other hand, P-C film promoted osteoblast to enter the differentiation stage, indicated by higher alkaline phosphatase activity and calcium deposition. In addition, the mean pore size of P-C film ranged at 7~9 μm, which was small enough to block the migration of cells through the membrane.
In conclusions, P-C film does not affect cell growth, and is able to promote the differentiation of osteoblast. Results of in vitro experiments indicate that P-C film has great potential in the applications of guided bone regeneration and bone tissue engineering.
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