THE STUDY OF DOUBLE-LAYER SURFACE MODIFICATION OF POROUS EPTFE FILMS BY PLASMA TREATED AND THE EFFECTS ON THE GROWTH OF CELLS

• Main Authors: Ruo-Yu Chen, 陳若語
• Other Authors: Chung-Yih Wang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/98575146554730574139

碩士 === 大同大學 === 生物工程學系(所) === 100 === Expanded polytetrafluoroethylene (ePTFE) films, was treated with argon plasma to improre the hydrophilicity. To create active site on the surface of ePTFE films, the coupling reaction with poly(ethylene glycol) methacrylate (PEGMA) or acrylamid (AAm) was initiated with UV. The contact angle was improved from 135°to 0°for modified ePTFE film with grafting poly(ethylene glycol) methacrylate (PEGMA；10wt%, and 30 minutes). The surface properties of ePTFE-g-PEGMA films and ePTFE-g-AAM films were also characterized by electron spectroscopy for chemical analysis, fourier transform infrared spectroscopy and scanning electron microscope. Peptide cRRE and collagen was crosslinked with (N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide)(EDC 5w%) on ePTFE-g-PEGMA films and ePTFE-g-AAM films. Human umbilical vein endothelial cells on one side of the ePTFE-g-AAm fixed polypeptide chain cRRE and ePTFE-g-AAm fixed collagen of the surface of HUVECs attached to the better,on the other hand the surface of human fibroblasts there is no better attachment rate of change menbrane, increasing the culture days to one weeks, better attached to the ePTFE-g-AAm and ePTFE-g-PEGMA fixed collagen rate in order to establish this modification in the field of biomedical applications. Different cells have different growth cycles, some the cell growth pay attention to develop the number of days in the double-sided .That can be enhance the biocompatibility of the film.