Fabrication of Hollow Gold Nanoparticle-Supported Lipid Bilayer for Drug Delivery

• Main Authors: Ming-Chih Tsai, 蔡明志
• Other Authors: Arthur Chiou
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/11152782266295176903

碩士 === 國立陽明大學 === 生醫光電研究所 === 100 === 　　This study is mainly to develop a silver template mediated gold nanoparticle for drug delivery with hollow interior, porous wall, and cell membrane-mimic composition supported surface. 　　To achieve the material behavior as describe, the process consists of three parts. The first part is to synthesis silver cores under different AgNO3 concentration. The UV/Vis absorption spectrums were acquired during reaction showed the nucleation of silver atom with time and different sizes of these particles. The following part is replacing silver core as templates into gold shells via galvanic replacement. Plasmon absorbance measured by UV/Vis spectroscopy indicated different states of silver core, silver/gold alloy, and complete gold shell. The final part is supporting lipid bilayer into shell surfaces, transmission electron microscopy was used to directly visualize these particles with or without lipid. 　　Here we report the plasmon resonance of hollow gold nanoparticles in infrared region as our major material and support with lipid for drug delivery. The examination of toxicity to Human lung cancer cell line CL1-0 was reached by redox assay. And the study of immune response with Maurine macrophage cell line J774.A1 was achieved via Griess reaction and viability assay. Loading of drug is based on pH gradient, rhodamine 6G dye or anticancer drug doxorubicin were loaded into carrier. Both trapping efficiency and release amount can be determined by fluorescence spectroscopy. Also these composites were applied to Human lung cancer cell line CL1-0 and following acquisition the images with a confocal microscopy.