| Summary: | 碩士 === 國立陽明大學 === 口腔生物研究所 === 100 === Owing to the poor prognosis, high mortality rate, and worldwide distribution, oral cancer is an important medical issue. More than 90% of oral cancer are oral squamous cell carcinoma (OSCC). To investigate the molecular alteration of OSCC can elucidate important diagnostic marker or therapeutic target. MicroRNAs can repress translations via imperfect binding on the 3’-UTR of target genes through seed sequence. Over 30% human genes are predicted to be regulated by microRNAs. In addition, microRNAs have been proved to play important roles in OSCC. In previous study, miR-187* was found highly expressed in OSCC and gastric cancer, however signaling pathways of miR-187* expression are still unknown. This study intend to figure out the role of miR-187* in oral carcinogenesis and to validate miR-187* as a potential biomarker of OSCC. First of all, I determined the expression of miR-187* in OSCC cell lines, tissue, plasma and saliva of oral cancer patient. It was found that miR-187* had higher expression level in OSCC cells and the tumor tissues than that in normal ones, but there were no differences between cancer and normal in saliva and plasma. Then, I investigated the effect of miR-187* overexpression on OSCC cell phenotypes including SAS and OECM-1 cell lines. Following miR-187* overexpression, the proliferation, migration and colony formation abilities were increased in OSCC cells, except for the proliferation of OECM-1 cells. The results suggest that miR-187* might be associated with metastasis. Third, I used miR-walk website and microcosm website and predicted SPRED1 and RB1 as predicted target genes of miR-187*. However, the preliminary analysis did not support that these genes were direct target genes of miR-187*. Besides, EMT markers and the phosphorylation of Akt and Erk were not affected by miR-187*. The role of miR-187* as a potential clinical biomarker of OSCC needed to be farther specified by analysis of more samples and by mechanistic elucidation.
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