The Study of Heat shock protein 27 and Oxidative Stress in Hemodialysis Patients

• Main Authors: Ma Hui-We, 馬慧玟
• Other Authors: Wang Hai-Lung
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/jfsbm9

碩士 === 元培科技大學 === 醫學檢驗生物技術研究所 === 100 === Patients with chronic kidney disease (CKD) by long-term hemodialysis (HD) treatment are significantly higher mortality and morbidity than the general population.Chronic inflammation, immune system activation, and enhanced oxidative stress are characteristic features of chronic kidney dieaasas.Heat shock protein 27 (HSP27) is a multidimensional protein which acts as a protein chaperone and an antioxidant and plays a role in the inhibition of apoptosis and actin cytoskeletal remodeling. In each of these capacities, HSP27 has been implicated in different disease states playing both protective and counter-protective roles.The current review presents HSP27 in multiple disease contexts: renal injury and fibrosis, cancer, neurodegenerative(including Alzheimer’s disease and Parkinson’s disease) and cardiovascular disease, highlighting its role as a potential biomarker and therapeutic target.Chronic kidney diseases are divided in five stages by eGFR; End stage renal disease(ESRD) should be subject to elongated treatment of hemodialysis to stay alive because his kidney is only 15% functional. This study has collected 128 blood samples from hemodialysis and 60 blood samples from non-hemodialysis to conduct blood routine test and related biochemical tests (BUN, Creatinine, Potassium, Calcium, Glucose AC, ALT/GPT, Alk-Phosphate,Total protein and Albumin) as well as oxidative stress analyses: Heat shock protein 27 (HSP27), Malondialdehyde (MDA) and Superoxide dismutase (SOD).The results showed that RBC count, Hemoglobin, Hematocrit, MCHC, Lymphocyte, ALT/GPT, Total Protein and Albumin were significant decreased in HD patients compared with non-HD subjects. WBC count, MCH, MCV, Neutrophil, Eosinophil, BUN, Creatinine, Potassium, Calcium, Gloucose and Alk-Phosphate were significan increased (p<0.05) in HD.Oxidative stress markers HSP27 (661.30±1114.46 vs 9.33±51.12 ng/mL, p<0.0001) and SODs (5.06±0.72 vs 3.13±0.89 U/L, p<0.0001) were significant increased in HD (p<0.05). MDA(5.29±4.95 vs 5.79±6.37 μmol/L,p=0.655 ) were no significant difference between the HD patients and the non-HD subjects (p>0.05).