Effect of the Different Conditions on Hydrogen Production by Co-cultured and Immobilized Photo- and Dark-fermentation Bacteria

• Main Authors: Chang, Chia-Min, 張家銘
• Other Authors: Hsu, Bin-Mu
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/10175395527794660085

碩士 === 國立中正大學 === 應用地球物理研究所 === 101 === In this study, bio-hydrogen production by fermentation was used. The result demonstrated that cumulative hydrogen formation (CHF=514ml), hydrogen yield (HY=1.97 mol H2/mol sucrose) and hydrogen production rate (HPR=0.0171 ml H2/ml/hr) with co-culture of R. palustris and C.butyricum were higher than R. palustris and C.butyricum in pure culture. A comparison of free cell and immobilized cell, immobilized cell’s CHF was 18% - 37% higher than free cell. HY and HPR were 53% - 80% and 36% - 58% higher than free cell, respectively, when using immobilized cell. The highest CHF (705ml), HY (3.52 mol H2/mol sucrose) and HPR (0.0270 ml H2/ml/hr) were obtained with the D/L ratio of 1/5 with immobilized cell. At different sucrose concentration CHF increased with increasing sucrose concentration from 4.45 g/L to 17.8 g/L and reached the maximum (705ml). HPR also increased with increasing sucrose concentration up to 35.6 g/L and reached the maximum (0.0292 ml H2/ml/hr). HY decreased from 5.42mol H2/mol sucrose to 3.01mol H2/mol sucrose when the sucrose concentration increased from 4.45 g/L to 35.6 g/L. Initial pH was varied from 5 to 8. CHF, HY and HPR all increased with increasing initial pH up to 8 and reached the maximum (830ml, 3.75 mol H2/mol sucrose, 0.0315 ml H2/ml/hr). Mixed the two kinds of medium (medium1 and medium2) in different ratio was studied. The ratio of medium1 and medium2 was varied between 1/0 and 0/1. CHF, HY and HPR all increased with decreasing medium1/medium2 ratio. The highest CHF (759ml) was obtained with medium1/medium2 ratio of 1/5. The highest HY (3.97mol H2/mol sucrose) was obtained with medium1/medium2 ratio of 1/2. The highest HPR (0.0283 ml H2/ml/hr was obtained with medium1/medium2 ratio of 1/5. At different amount of immobilized cell, CHF and HPR increased with increasing amount of immobilized cell. HY was varied between 2.73mol H2/mol sucrose and 3.52 mol H2/mol sucrose. TVFA（totally volatile fatty acids）concentrations increased with increasing initial sucrose concentrations. The end pH decreased with increasing TVFA concentrations. Not only TVFA concentrations but also buffer would control the end pH. Moreover, different of initial sucrose concentration and initial pH would affect the kinds of VFAs which were formation from dark fermentation. All of these factors would affect hydrogen formation. Gompertz equation was used as the kinetic model in this study. CHF from Gompertz equation were much higher than experiment data. These results suggested that using Gompertz equation as the kinetic model in this study needed to modify to fit the experiment data.