| Summary: | 碩士 === 長庚大學 === 生化與生醫工程研究所 === 101 === Negative selection-based circulating tumor cells (CTCs) isolation method can harvest the CTCs in a label-free manner that is valuable for the subsequent CTCs-based assays. However, such cell isolation schemes normally suffer from the low CTC purity. To address the issue, the utilization of optically-induced dielectrophoretic (ODEP) force in a microfluidic chip to further purify the CTCs was proposed. The working principle is primarily based on the operation conditions of ODEP for the manipulation of CTCs (prostate cancer 3 and human oral cancer as tested cells) and leukocytes are different due to the size difference between the two cells. By fine-tuning the operation conditions of ODEP force, cancer cells can be isolated from a blood cell background in an effective manner. In this study, operation conditions of ODEP for the manipulation the cells were first investigated. The isolations of cancer cells from cell mixtures with different cancer cell purity were carried out. Results revealed that the obtained cancer cells were viable, indicating that such CTC isolation process is cell-friendly. Moreover, the proposed CTC isolation scheme was proved to be able to isolate cancer cells from the cell mixture with high recovery rate (76%-83%), and high purity (74%-82%). As a whole, this study has presented an efficient and effective method for further enhancing the purity of CTCs after the conventional CTC isolation process.
|
|---|
