Bioengineered dermal fibroblast spheroids fabricated from hyaluronic acid coatings for corneal stromal reconstruction
碩士 === 長庚大學 === 生化與生醫工程研究所 === 101 === Corneal stromal cells are not readily accessible, and it is difficult to obtain sufficient number of cells from patients. Therefore, an alternative strategy for corneal stromal reconstruction should be established to meet the increasing medical service needs. H...
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| Format: | Others |
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2013
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| Online Access: | http://ndltd.ncl.edu.tw/handle/22241669856397169832 |
| Summary: | 碩士 === 長庚大學 === 生化與生醫工程研究所 === 101 === Corneal stromal cells are not readily accessible, and it is difficult to obtain sufficient number of cells from patients. Therefore, an alternative strategy for corneal stromal reconstruction should be established to meet the increasing medical service needs. Here, we demonstrate the feasibility of cultivating dermal fibroblast spheroids on biomaterial coatings and transplanting these cell grafts to treat corneal wound.
To overcome the drawbacks associated with shortage of allograft, this study aims to develop bioengineered dermal fibroblast spheroids for corneal stromal tissue reconstruction.Rabbit dermal fibroblast (RDF) spheroids were fabricated on hyaluronic acid (HA) coatings and were evaluated by determinations of cell proliferation and extracellular matrix production capacity. The multicellular spheroid aggregates were implanted in a rabbit model of bacterial keratitis and the postoperative outcomes were evaluated by means of clinical observations including slit-lamp biomicroscopy and pachymetry. Animals receiving dissociated cell suspensions harvested from tissue culture polystyrene (TCPS) plates were included for comparison.
Our results showed that the RDFs on HA coatings exhibited poor adhesion and tended to aggregate to form spheres. When compared to the TCPS plates, the HA-coated substrates significantly hindered cell spreading and subsequent cell proliferation. The RDF spheroids possessed better biosynthetic capacity and tissue repair than their counterparts in the form of dissociated cell suspensions. The injection of multicellular spheroid aggregates into the stromal defect could improve corneal clarity and edema. Bioengineered RDF spheroids may have potential for use in corneal stromal regenerative medicine.
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