Ameliorative effects of Dendrobium tosaense extracts on osteoporosis in ovariectomized rats.
碩士 === 中國醫藥大學 === 基礎醫學研究所碩士班 === 101 === The present study evaluated the prebiotic effect of Dendrobium tosaense extracts (DT) and its effects on osteoporosis in ovariectomized (OVX) rats. The OVX rats were randomly divided into four groups and orally treated with water, DT (300 and 900 mg/kg daily)...
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ndltd-TW-101CMCH53250022016-03-21T04:27:53Z http://ndltd.ncl.edu.tw/handle/99881864776500526755 Ameliorative effects of Dendrobium tosaense extracts on osteoporosis in ovariectomized rats. 黃花石斛粗萃物對大鼠去卵巢誘發骨質疏鬆的改善效果 Jyun-Jhih Wang 王俊智 碩士 中國醫藥大學 基礎醫學研究所碩士班 101 The present study evaluated the prebiotic effect of Dendrobium tosaense extracts (DT) and its effects on osteoporosis in ovariectomized (OVX) rats. The OVX rats were randomly divided into four groups and orally treated with water, DT (300 and 900 mg/kg daily) and inulin (400 mg/kg daily) for 14 weeks. The sham group was orally treated with water. The DT treatment enhanced the number of faecal bifidobacteria in OVX rats. The results of a Ca-balance experiment showed that DT increased apparent Ca absorption and retention. The OVX rats were killed after DT treatment lasting 12 weeks. The mineral content, density and biomechanical strength of bones were lower in OVX rats than the sham group, but these bone loses were prevented by DT administration. Microtomography scanning showed that the DT-treated rats had higher trabecular bone volume than the OVX . In vitro study demonstrated that DTM100 fraction from DT inhibited osteoclast differentiation. Western blot was used to analyze osteoclast-associated signaling pathway in RAW264.7 cells. Results showed that DTM100 inhibits the RANKL-triggered nuclear translocation of NF-κB and nuclear factor of activated T cells c1 (NFATc1). RT-PCR was used to analyze osteoclast resorption-associated gene expression in RAW 264.7 cells. DTM100 inhibits the expression of cathepsin K and TRAP. In conclusion, In vivo studies provide evidence that prebiotic activity might be involved in the anti-osteoporotic mechanisms of DT. In addition, in vitro study also showed that DT could directly inhibit osteoclast differentiation. DT could be a potential candidate for the treatment of post-menopausal osteoporosis. Wen-Chuan Lin 林文川 2013 學位論文 ; thesis 82 zh-TW |
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碩士 === 中國醫藥大學 === 基礎醫學研究所碩士班 === 101 === The present study evaluated the prebiotic effect of Dendrobium tosaense extracts (DT) and its effects on osteoporosis in ovariectomized (OVX) rats. The OVX rats were randomly divided into four groups and orally treated with water, DT (300 and 900 mg/kg daily) and inulin (400 mg/kg daily) for 14 weeks. The sham group was orally treated with water. The DT treatment enhanced the number of faecal bifidobacteria in OVX rats. The results of a Ca-balance experiment showed that DT increased apparent Ca absorption and retention. The OVX rats were killed after DT treatment lasting 12 weeks. The mineral content, density and biomechanical strength of bones were lower in OVX rats than the sham group, but these bone loses were prevented by DT administration. Microtomography scanning showed that the DT-treated rats had higher trabecular bone volume than the OVX .
In vitro study demonstrated that DTM100 fraction from DT inhibited osteoclast differentiation. Western blot was used to analyze osteoclast-associated signaling pathway in RAW264.7 cells. Results showed that DTM100 inhibits the RANKL-triggered nuclear translocation of NF-κB and nuclear factor of activated T cells c1 (NFATc1). RT-PCR was used to analyze osteoclast resorption-associated gene expression in RAW 264.7 cells. DTM100 inhibits the expression of cathepsin K and TRAP.
In conclusion, In vivo studies provide evidence that prebiotic activity might be involved in the anti-osteoporotic mechanisms of DT. In addition, in vitro study also showed that DT could directly inhibit osteoclast differentiation. DT could be a potential candidate for the treatment of post-menopausal osteoporosis.
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author2 |
Wen-Chuan Lin |
author_facet |
Wen-Chuan Lin Jyun-Jhih Wang 王俊智 |
author |
Jyun-Jhih Wang 王俊智 |
spellingShingle |
Jyun-Jhih Wang 王俊智 Ameliorative effects of Dendrobium tosaense extracts on osteoporosis in ovariectomized rats. |
author_sort |
Jyun-Jhih Wang |
title |
Ameliorative effects of Dendrobium tosaense extracts on osteoporosis in ovariectomized rats. |
title_short |
Ameliorative effects of Dendrobium tosaense extracts on osteoporosis in ovariectomized rats. |
title_full |
Ameliorative effects of Dendrobium tosaense extracts on osteoporosis in ovariectomized rats. |
title_fullStr |
Ameliorative effects of Dendrobium tosaense extracts on osteoporosis in ovariectomized rats. |
title_full_unstemmed |
Ameliorative effects of Dendrobium tosaense extracts on osteoporosis in ovariectomized rats. |
title_sort |
ameliorative effects of dendrobium tosaense extracts on osteoporosis in ovariectomized rats. |
publishDate |
2013 |
url |
http://ndltd.ncl.edu.tw/handle/99881864776500526755 |
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