| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技系 === 101 === Helicobacter pylori can infect human’s stomach and cause gastric ulcer and duodenal ulcer. Moreover, it is intimately related to gastric cancer. Currently, antibiotics combined with proton pump inhibitor (PPI) are the major treatment. However, antibiotic treatment still requires to be improved due to the problem of drug resistance and the side effects. Lactobacilli have been used to improve the intestinal malfunction and diseases which included the H. pylori infection. Howerer, different genes and species may have different effects. This study aimed to explore lactic bacteria’s capability in inhibiting Helicobacter pylori. In order to find out high antimicrobial capability of lactic bacteria, Synbiotech Inc. Provided 50 lactic bacterial strains for this research. It was found that lactic acid bacteria 11, lactic bacteria 33 and lactic bacteria 37 can inhibit not only the common H. pylori but also multidrug resistance of H. pylori under the atmosphere and microaerophilic conditions. The concentrated supernatant of lactic bacteria inhibited the growth of H. pylori, and the effect of inhibition was better than currently commercial lactic bacteria. Destroyed protein components of culture supernatant through heat or change pH of culture media confirmed three lactic bacteria can secrete lactic acid to inhibit H. pylori. These lactic bacteria can adhere in AGS and MKN45 cell lines, suggesting they are able to stay in stomach. However, adding these lactic bacteria cannot prevent H. pylori from being adhered in AGS and MKN45 cell lines. Hence, there is a big possibility that lactic bacteria strains might not possess antibacterial ability and reduce the expression of IL-8. In addition, they do not appear to interfere with human immune response. In the animal model, we studied on the influence
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between H. pylori strain 625 and lactic bacteria strains in mouse. After being infected by H. pylori, these lactic bacteria strains appear to decrease acute inflammation and chronic inflammation in body, antrum and whole stomach within three to six weeks. Based on this study, it is reasonable to assume lactic bacteria 11, lactic bacteria 33 and lactic bacteria 37 possess the ability to decrease the H. pylori infection.
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