The Application of Taiwan Tea on Cosmetic Products

• Main Authors: An-Ni Hsu, 許安妮
• Other Authors: Zong-Shiow Chen
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/26793602188438424532

碩士 === 嘉南藥理科技大學 === 化粧品應用與管理系暨化粧品科技研究所 === 101 === In our present study, we used Tea R (Camellia sinensis) and Tea S (Camellia transarisanensis) were planted in Taiwan Nantou, used water and 50% alcohol as the solvent, and the company prvided propylene glycol as solvent with heating circumfluence extraction, than decompress concentrating, freeze-dried. Took the tea extracts conducted to in vivo efficacy assessment, MTT assay and HPLC component analysis, applied in the development of cosmetic products, and then the stability of the products and efficacy assessment. Our results indicated that the Tea R extracts with water as for radical scavenging DPPH and ABTS, had strong scanenging, respectively SC50 is 0.016±0.0002 mg/ml and 0.007±0.0006 mg/ml. Tea S extracts with 50% ethanol as for radical scavenging DPPH and ABTS, respectively SC50 is .017±0.002 mg/ml及0.006±0.001 mg/ml. However, statistical analysis showed that Tea S extracts with water and 50% ethanol on DPPH ˙ radical scavenging and ABTS ˙ + radical scavenging rate was not significantly (P> 0.05). Tea R water extract (IC50=0.074±0.038 mg/ml) has antimelanogenic the better than L-ascorbic acid (IC50=0.09 mg/ml). Determination of total phenolic content, the first is Tea S extracts with 50% ethanol (252.19 mg GAE/g), the second is the Tea R water extract(158.80 mg GAE/g). pUC 119 DNA protention experiment, at 0.125 mg/ml , Tea R and Tea S with three extract protection the pUC119 DNA. MTT assay, at 0.05 mg/ml, Tea R and Tea S with three extract, make 3T3 fibroblast survival rate more than 60%. HPLC analysis, Tea R and Tea S with three extract, contains Catechin, (-)Epicatechin, Chlorogenic acid, Caffeic acid, Tea S 50% ethanol extract is the most abundant compound with about 1.834 mg/ml (-)Epicatechin on basis. In vivo efficacy assessment, add to Tea R extract can take upgrade in skin bright (L value), and improved from an average of 63.73 to 72.37, improvement rate was 10.1 %, so Tea R extract can development and application on cosmetic products.