Osteogenic and immunogenesis potentials of co-cultured osteoblast cells and pre-osteoclast cells on calcium silicate cement

• Main Authors: Wei-Yun Lai, 賴威勻
• Other Authors: 黃翠賢
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/66520474769188365070

碩士 === 中山醫學大學 === 牙醫學系碩士班 === 101 === It is acknowledged that MTA has been one of the most significant developments in the endodontic field during the past 20 years. As MTA is characterized by superior physical, chemical properties along with biocompatibility, it is mainly used for perforation repair, root-end filling, and pulp capping related therapy. Our laboratory has also developed a novel calcium silicate cement (CS cement) with a similar composition and nature to the MTA. When in clinical application, these materials often directly contact with periodontal tissue and alveolar bone. Hence, the influence and relationship between the material and bone cell require an in-depth understanding. In this sense, the purpose of this study aims to investigate Osteogenic and immunogenesis potential of co-cultured osteoblast cells and pre-osteoclast cells on calcium silicate cement. This study will employ cell compatibility test, cell cycle test, SEM, ion releasing test and western blotting to compare cell proliferation and inflammation between CS and MTA. Tissue culture plate was used as control. Results indicate that CS contributes greatly to stimulation for proliferation of osteoblast, while CS has an inhibiting effect on pre-osteoclast cells growth by showing statistical significance between CS and TCP(P<0.05). MTA also helps stimulate the proliferation of osteoblasts but no significant effect on pre-osteoclasts. SEM images show that osteoblast cells perform great cell adhesion on the surface of CS and MTA, while pre-osteoclast cells only adhere to the surface of MTA. In the experiments regarding inflammatory factors and osteogenic related facors, results reveal that CS can inhibit inflammation (TNF-α、iNOS、COX-2) by showing significant difference between CS and TCP (P<0.05), and restrain pre-osteoclast differentiation by stimulating osteoblast proliferation to increase OPG secretion which is competitive to RANKL for RANK.