Study of the plasma Monocyte Chemoattractant Ptotein-1 levels in association with the severity in Ischemic Stroke patients

• Main Authors: Yu-Ting Wang, 王瑜婷
• Other Authors: Chun-Wen Cheng
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/78076600266116761959

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 101 === Ischemic strokes occur as a result of an obstruction within a blood vessel supplying blood to the brain. Two main causes for ischemic stroke are cerebral thrombosis and cerebral embolism. If can predict the occurrence of stroke, reduce the incidence of stroke and reduce the severity of stroke will also reduce medical resources and the burden on family. Chemokine-driven migration of inflammatory cells has been implicated in pathogenesis of atherosclerosis-associated conditions such as ischemic stroke and myocardial infarction. It is well established that cerebral ischemia results in increased expression of several cytokines and chemokines that precedes infiltration of leukocytes into the ischemic lesion. These leukocytes contribute to tissue injury in ischemic stroke. Individuals with symptomatic carotid atherosclerotic plaque have high risk of ischemic stroke. Previous studies have shown an association between MCP-1 levels and the incidence of ischemic stroke. In the study, investigate plasma MCP-1 levels and the severity ischemic stroke , we speculate that the increase in MCP-1 may increase the severity of ischemic stroke.There, venous blood from 335 human subjects was collected, 220 blood samples of which were obtained from patients with stroke, and 115 healthy controls who had no history of serious illness. Plasma MCP-1 levels were measured using enzyme-linked immunosorbent assay. We also correlated the data clinically and carried out risk factor analysis based on the detailed questionnaire obtained from each patient. In comparison to control subject (control mean:0.41±0.19ng/ml), MCP-1 plasma levels were increased in stroke patient (stroke mean: 0.43±0.20ng/ml), have higher correlated was between control and stroke(P=0.04). That form severity analysis, it was ovserved not association with severity in patients. That form gender analysis, with significant correlation (P =0.02), case male than control men. Similarly, no relationship was ovserved between chemokine levels stroke risk factors(hypertension, diabetesetc), Analysis showed that hypertension , smoking, and alcohol consumption correlated and MCP-1 was also not related to gender. Conclusion: The incidence of ischemic stroke and plasma MCP-1 concentrations are related, but could not confirm MCP-1 and ischemic stroke the severity of correlated.