| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 101 === Breast cancer is the most common cancer in women in the Taiwan. Then breast cancer deaths accounted for 28.4% of all the cancer deaths, and have been associated with obesity. Previous studies, has been confirmed Nelumbo nucifera leave extract (NLE) can effectively reduce the fatty liver, atherosclerosis, hyperlipidemia and anti-oxidative properties. Thus, we investigated the relationship between obesity and breast cancer, and evaluated the inhibitory effect of NLE on the adipocytes-induced breast cancer progression. Adipocyte secreted conditioned medium (CM) noticeably induced breast cancer migration. NLE diminished the CM-induced migration number of breast cancer cell by boyden chamber assay. A direct correlation was found between adipocyte and breast cancer cell migration. Similarly, we used of ECIS assay, wound healing assay and transwell assay identical results were found. To investigate the molecular mechanisms of breast cancer cell migration using two-dimensional gel electrophoresis. We found CM increased the proteins level of the Hsp90, StarD10, Peroxiredoxin 6. The results suggest that CM stimulates migration of breast cancer cells, perhaps through the interaction of these proteins. Under the CM treatment, phosphorylated JNK, PI3K/Akt, StarD10 and Peroxiredoxin 6 levels of MCF-7 cells treated with NLE can significantly inhibited the protein expression were measured by Western blot analysis. These data indicate adipocytes provide a major component of the microenvironment for rapid tumor growth and migration, and this increase was inhibited significantly by NLE treatment. In summary, the study indicated that the future for the treatment of metastatic breast cancer, NLE inhibited cancer metastasis has developed into a process of natural drugs.
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