| Summary: | 碩士 === 中山醫學大學 === 醫學檢驗暨生物技術學系碩士班 === 101 === Obesity is a chronic and low-grade inflammation, which can cause insulin resistance and affect the functions of adipocytes. Different types of macrophages (M1 or M2) may be involved in the metabolism of adipose tissue. In our previous studies, the conditioned medium from anti-inflammatory macrophages (M2) can affect the 3T3-L1 adipocytes differentiation and decrease the quantity of lipid droplets inside of adipocytes but the adipocyte differentiation marker (PPAR-γ, AP-2) had no significant change. Next, we focused on Fat-specific protein 27 (FSP27) and perilipin, both are lipid-droplet coating proteins and correlated with oil droplet formation and lipolysis. The results showed that the mRNA and protein level of perilipin are both decreased in the presence of M2-conditioned medium. It is proposed the M2-conditioned medium affects the expression of oil droplets coated protein or the process of droplet formation, leading to the reduction of mature adipose tissue. Our data also showed that, glycerol, the end product of lipolysis, were also decreased in the M2-conditioned medium group. However, the size of oil droplet seems to be larger in M2-conditioned medium group compared with positive group under microscopic observation. The detail molecular mechanism of this phenomenon is under further investigation. In summary, the M2-conditioned medium group are effectively reduced the droplet forming and lipid metabolism.
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