| Summary: | 碩士 === 逢甲大學 === 化學工程學系 === 101 === In this study, ITO glass as a substrate and using constant potential method on ITO glass coated with ZnO film to increase its electrical conductivity. after that, we use self-assembled technology at the 3-aminopropyltrimethoxysilane (APTMS) coated on the ZnO film and use the covalent bonding method that to form amide bond between urease and amino group on the sensor electrode. Pt-C catalyst was added to enhance potential signal. The enzyme electrode to be detect in electrochemical sensing system. Looking for the best preparation conditions for the enzyme electrode. Finally, the performances of the enzyme electrodes made from different temperature and different pH were examined and compared.
According to the experimental results, urease concentration of 80 mg/ml makes the best enzyme electrode. At T = 50℃, pH = 7 environment, with the best sensitivity 43.600 mV / decade. After two weeks the enzyme activity of the biosensor still 75%.
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