Developing of Biochemistry Assays Based on a Microfluidic Disk Analyzer

• Main Authors: LIN,CHIA-HUI, 林佳慧
• Other Authors: 施志欣
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/99562508794580875810

博士 === 逢甲大學 === 化學工程學系 === 101 === This study investigated microfluidic functions on the centrifugal platform including supernatant decanting, which is related to deformation &; recovery of the plastic lids, rapid mixing, and liquid replacement. It was found that the deformed volume is proportional to the square of the rotational speed, and the average radius of liquid, and inversely proportional to the cube of the thickness of the chamber lids. As for the decanted volume, it is related to the recovered volume of the deformed chamber lids, the inertial force resulted from the deceleration of the rotational speed during decanting process and the flow resistance in the decanting device. 3D decanting design demonstrated better volume control than 2D decanting design especially when fluids with various viscosities were used. In addition, a fast and effective micromixer based on a meandering channel on a centrifugal platform was developed in this study. The liquid replacement mechanism in the detection chamber was also investigated. It was found that low inlet flowrate resulted in better liquid replacement in the detection chamber. Finally, a fully integrated prothrombin time test is presented in this study. By integrating the microfluidic functions mentioned above, the microfluidic disc is able to extract the plasma from whole blood samples, and conduct rapid mixing within 1 second. Clinical tests were conducted for 92 patients by this lab-grade analyzer and the results showed that there was no significant difference between our lab-grade analyzer and the instruments used in the local hospital.