| Summary: | 碩士 === 輔仁大學 === 營養科學系碩士班 === 101 === Aluminum (Al) salt as common adjuvant makes the vaccine more effective to enhance immune response. Some reports have shown that certain infant formulas contain high concentrations of Al, but the absorbed Al might not sufficiently excreted in neonates who have immature kidneys. Aluminum may directly affect the immune response by accumulation in human’s organ and tissue, especially in immune tissue. On the other hand, maltol can chelate metals and has a high affinity with Al, which may enhance the Al absorption in the gastrointestinal tract. Therefore, this study was conducted to understand the effects of Al maltolate on immune system of neonates, and whether those animals with higher plasma and/or tissues Al levels are more susceptible to specific antigen. Three-day-old breast-fed pups were divided into four groups with gavage twice a day of 0 (Control, C), 0 (Maltolate, M), 1.3 (low Al maltolate, LAL) and 3.9 (high Al maltolate, HAL) μg Al/g b.wt /day respectively for 15 days. OVA/TiterMax Gold adjuvant-immunization were intraperitoneally injected to neonates at 5-day of age. At the age of 19-day, animals were sacrificed, followed by collection of blood, liver, thymus, spleen and mesenteric lymph node (MLN) for analysis. Cell proliferation and cytokine concentration of thymocyte, splenocyte and MLN lymphocyte were measured. Results showed that there was no tendency of Al content in tissue elevated with increased dietary Al maltolate. In the systemic immunity, the level of IgG was significantly lowest in Al group which dietary Al levels were equivalents to 1 to 3 times of infant formula. Animals of high Al group had significantly higher Con A-stimulated cell proliferation, and Interlukin-5 (IL-5) of splenocytes. However, there was no significant difference in cell proliferation of thymocyte among 4 groups. On the other hand, the secretion of IL-2 by MLN cell was significant lowest in low Al group. IgA levels in homogenized intestine fluid were not significant difference among 4 groups. In conclusion, dietary Al maltolate with Al levels equivalents to 1 to 3 times of infant formula affect immune response by changing splenocyte proliferation and secretion of cytokine. Further study needed to see the effect of combination of different dietary Al and maltose levels on the Al levels in vivo.
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