Study of the Innate Immunity and Antioxidant Effects of Sulforaphane in C57BL/6 Mice

• Main Authors: Hsu, Tze-Ling, 徐慈玲
• Other Authors: Wu, Wen-Mein
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/32538707494967243992

碩士 === 輔仁大學 === 營養科學系碩士班 === 101 === Ageing-related chronic diseases usually accompany with increased oxidative stress and retared immunity. The precursor of sulforaphane (SFN) is found in rich quantities in crucifer, and it can be transformed into SFN by myrosinase. Many studies suggested that SFN possess anti-carcinogenic and anti-inflammatory effects. In this study, we would like to explore the possibility of immune and antioxidant effects of SFN on C57BL/6 mice. We divided the study into two parts. In part one study, young C57BL/6 mice were divided into three groups, and gavaged with deionized water, low or high dose SFN (0.2 or 1.0 mg/ Kg BW) SFN for 14 days, respectively. After mice been sacrificed, the cytokine secretion pattern, cell proliferation ability, phagocytosis activity, natural killer cell cytotoxicity activity of the splenocytes, peritoneal exudate cells (PEC) involced proinflammatory response, and the liver antioxidant enzyme activity were analyzed. In the second part of study, the mice will be induced aging by s.c. injected with 1 % D-galactose (DG). The C57BL/6 mice divided into four groups: the normal control group, the aging control group (AC group) and two other aging group been fed with SFN, low or high dose (0.2 or 1.0 mg/ Kg BW) SFN for 8 weeks. The first part’s results suggested that SFN could significantly decreased the secretory levels of nitric oxide (NO) and prostaglandin E2 (PGE2) from PEC co-cultured with LPS/IFN-γ and also significantly increased the IFN-γ secretion of splenocytes, and the phagocytosis activity as compared to the control group. The inflammatory cytokines (IL-6 and TNF-α) secretion of PEC and splenocytes had less tendencies in SFN-fed groups. Mice been short term fed with SFN could also enhanced the glutathione peroxidase activity in liver (p<0.05). In the second part of study, we found that mice been fed with SFN could enhance the IFN-γ secretion by splenocytes, and elevated both the phagocytosis activity and the nature killer cell cytotoxity ability as compared to the AC group (p<0.05). Besides, the inflammatory related mediators (such as: NO and PGE2) secreted by PEC and TNF-α secreted by PEC and splenocytes had less tendencies in SFN-fed groups. In SFN-fed group, the antioxidant enzymes activity (glutathione peroxidase and superoxide dismutase) of liver and kidney were significantly improved as compared to the AC group. And the levels of liver protein carbonylation contents and plasma RAGE were significantly reduced in SFN-fed group as compared with the AC group. In summary, SFN may play a beneficial role on reducing the oxidative stress, improving the innate immunity and attenuating inflammation. There is a great potential for developing SFN as an anti-aging functional foods.