Role of Andrographis paniculata Extract in Neuroprotection
碩士 === 中華醫事科技大學 === 生物醫學研究所 === 100 === Andrographis paniculata Nees., Family Acanthaceae, is a traditional folk medicinal plants, widely used in various applications, and has been used as antiinflammatory, antibacterial, antipyretic and immune promoting traditional therapeutic drugs. Excessive rea...
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ndltd-TW-101HWAI71140032016-07-02T04:20:07Z http://ndltd.ncl.edu.tw/handle/30755644843011053902 Role of Andrographis paniculata Extract in Neuroprotection 穿心蓮萃取物在神經保護的角色 Yang, Shu-Fang 楊淑雱 碩士 中華醫事科技大學 生物醫學研究所 100 Andrographis paniculata Nees., Family Acanthaceae, is a traditional folk medicinal plants, widely used in various applications, and has been used as antiinflammatory, antibacterial, antipyretic and immune promoting traditional therapeutic drugs. Excessive reactive oxygen radicals produced by oxidative stress make the brain hurt easily. It has been known that oxidative stress could easily lead to the generation of neurodegenerative diseases and the extract of Andrographis paniculata is a good antioxidant. Therefore, we used the human neuroblastoma IMR-32 cells treated with hydrogen peroxide and ethanol oxidation to investigate whether Andrographis paniculata extract has antioxidant neuroprotective effects. Under the cellular experimental conditions, the use of hydrogen peroxide and ethanol can induce cell death. To study the protective effects of Andrographis paniculata extract, we treated cells with hydrogen peroxide/ethanol and various concentrations of Andrographis paniculata extract (25, 50, 100, 200 μg/ml) for 24 hours and found the extract can protect cells from death. We studied whether the cell death via apoptosis using ApoGlowTM assay kit and found that hydrogen peroxide/ethanol induces apoptosis and Andrographis paniculata extract decreases the adverse effects. Integrated our experimental results, Chinese herbal medicine Andrographis paniculata extract protects nerve cells against oxidative stress injury. Maybe it can be used for prevention or treatment of neurodegenerative diseases in the future. Chang, Wen-Teng 張文騰 2013 學位論文 ; thesis 51 zh-TW |
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碩士 === 中華醫事科技大學 === 生物醫學研究所 === 100 === Andrographis paniculata Nees., Family Acanthaceae, is a traditional folk medicinal plants, widely used in various applications, and has been used as antiinflammatory, antibacterial, antipyretic and immune promoting traditional therapeutic drugs. Excessive reactive oxygen radicals produced by oxidative stress make the brain hurt easily. It has been known that oxidative stress could easily lead to the generation of neurodegenerative diseases and the extract of Andrographis paniculata is a good antioxidant. Therefore, we used the human neuroblastoma IMR-32 cells treated with hydrogen peroxide and ethanol oxidation to investigate whether Andrographis paniculata extract has antioxidant neuroprotective effects. Under the cellular experimental conditions, the use of hydrogen peroxide and ethanol can induce cell death. To study the protective effects of Andrographis paniculata extract, we treated cells with hydrogen peroxide/ethanol and various concentrations of Andrographis paniculata extract (25, 50, 100, 200 μg/ml) for 24 hours and found the extract can protect cells from death. We studied whether the cell death via apoptosis using ApoGlowTM assay kit and found that hydrogen peroxide/ethanol induces apoptosis and Andrographis paniculata extract decreases the adverse effects. Integrated our experimental results, Chinese herbal medicine Andrographis paniculata extract protects nerve cells against oxidative stress injury. Maybe it can be used for prevention or treatment of neurodegenerative diseases in the future.
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author2 |
Chang, Wen-Teng |
author_facet |
Chang, Wen-Teng Yang, Shu-Fang 楊淑雱 |
author |
Yang, Shu-Fang 楊淑雱 |
spellingShingle |
Yang, Shu-Fang 楊淑雱 Role of Andrographis paniculata Extract in Neuroprotection |
author_sort |
Yang, Shu-Fang |
title |
Role of Andrographis paniculata Extract in Neuroprotection |
title_short |
Role of Andrographis paniculata Extract in Neuroprotection |
title_full |
Role of Andrographis paniculata Extract in Neuroprotection |
title_fullStr |
Role of Andrographis paniculata Extract in Neuroprotection |
title_full_unstemmed |
Role of Andrographis paniculata Extract in Neuroprotection |
title_sort |
role of andrographis paniculata extract in neuroprotection |
publishDate |
2013 |
url |
http://ndltd.ncl.edu.tw/handle/30755644843011053902 |
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