Silver nanoparticles induce heat shock response and provide protective effect in Clone 9 cells

• Main Authors: Chia-Ching Ho, 何佳靜
• Other Authors: Rei-Cheng Yang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/02079991482555130666

碩士 === 高雄醫學大學 === 生理及分子醫學研究所 === 101 === Silver nanoparticles (Ag-nps) are currently emerging as one of the fastest growing product categories in the nanotechnology industry by it’ s antimicrobial activity. As being found in other nano materials, it had been proved that silver nanoparticles has cytotoxicity in primary liver cells. However, silver nanoparticles have also been found to induce the expression of heat shock protein70 (Hsp70), which processes the cellular self-protective effect against subsequent lethal stress. In this study, we aimed to explore the potential role of Ag-nps-induced Hsp70 in protecting the cell death damaged by TNF-alpha. The results showed that clone 9 cells, a hepatic epithelial cell line, have an optimal over-expression of Hsp70 upon exposure to 10 μM of Ag-nps for 8 hours, without evidence of changes in the survival rate by MTT assay. After Ag-nps pretreatment, TNF-α was added as a cell death inducer to observe the protection of Hsp70. The protective effect was confirmed by the significantly increased survival rate in Ag-nps pretreated clone 9 cells. It was concluded that optimal dose of Ag-nps induced Hsp70 over-expression and exerted its protective function against proinflammatory cytokine-induced cell death. These results highlight the therapeutic potential of Ag-nps in inflammatory diseases.