Optical Fiber Immuno-sensor based on Long Period Fiber Grating

• Main Authors: Jia-Ying Huang, 黃嘉瑩
• Other Authors: Chia-Chin Chiang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/63007982310996093057

碩士 === 國立高雄應用科技大學 === 機械與精密工程研究所 === 101 === In this thesis, the Sandwiched long period fiber grating (SLPFG) and Box-shape long period fiber grating (Box-shape LPFG) were fabricated by thick photoresist stacking technique with SU-8 and etching process in Photolithography as the immuno-sensor. The fabrication makes SLPFG and Box-shape LPFG to obtain LPFG function effectively. First to silanition, the LPFG was immersed in NaOH solution and (3-Aminopropyl)triethoxys (APTES) solution to generate O-H bond and N-H bond on the surface of optical fiber for binding biomolecule. The spectrum were varied strongly when O-H bond binding N-H bond. For SLPFG, the resonant wavelength shift was 18.232nm, the transmission loss increased -2.31dB, and for Box-shape LPFG, the resonant wavelength shift was 0.999nm, and the transmission loss increased -2.66dB. Second to analyze the resonant wavelength and transmission loss for SLPFG and Box-shape LPFG that HDAC6 binding specificity secondary antibodies horseradish peroxidase on the surface of immune-sensors. For SLPFG, the resonant wavelength shift was 15.984nm and the transmission loss increased -12.73dB. For Box-shape LPFG, the resonant wavelength shift was 11.98nm and the transmission loss increased -7.6dB. For separating the cell membranes and impurities, the Mahlavu and SK-Hep1 cells were transferred with expression plasmids and the extraction were drew to the 1.5mL tube As the result shown in Mahlavu, the resonant wavelength shits were steady and transmission loss were -0.22dB. For SK-Hep1 cells, the resonant wavelength shits were 24.27nm and transmission loss were -8.92dB. In conclusion, this immuno-sensor for detecting specific protein for HDAC6 is workable.