Elucidation of CID and ETD based MS/MS Fragmentation for Analysis of Cyclic Peptides

• Main Authors: Jia-Hau Wu, 吳家豪
• Other Authors: Chien-Chen Lai
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/36605916615873499983

碩士 === 國立中興大學 === 分子生物學研究所 === 101 === The cyclic peptide is one kind of cyclic compounds which combine of the protein and non-protein amino acids, many of kinds has more different functions, such as antibodies, toxic substances, anti-immune substances, ion transfer regulators, protein binding inhibitor or enzyme inhibitors. The application of the cyclic peptide is quite diverse, and gradually attracted the attention of scholars. The research strategy of this experiment is based on electron-spray ionization tandem mass spectrometry (ESI-MS/MS) combine with collision induced dissociation (CID) and electron transfer dissociation (ETD) to sequence cyclic peptides, to apply to the determination of cyclic peptides, Surfactin、Colistin、Polymyxin B、Iturin a and Bacitracin. Observations of different amino acid residues from CID and ETD experiments for the peptide were interpreted by their fragments, and try to join with metal acetate solution, that expect to enhance the number of electric charge for cyclic peptide in the electrospray ionization method, and observe whether increases the sequence coverage of ETD or not, to improve the qualitative degree the establishment of a cyclic peptide analysis platform, it might be the related bacteria purified cyclic peptide technology development and quantitative research.