| Summary: | 碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 101 === Tumor (cancers) is one of the ten leading causes of death, and lung cancer is the main causes of cancer death worldwide. With the raising of modern herb medicine, more and more herbs are tested against cancers. Sapindus saponaria, native to warm temperate to tropical regions like the southern region of Yangtze river in China, India, Japan, Vietnam, Laos and Taiwan, is a well-known deciduous tree. The drupes can be used as a natural surfactant for cleaning. In MTT assay we found that the methanol extract of S. saponaria has effect to inhibit the growth of A549 NSCLC cell lines. The methanol extract of S. saponaria owns the ability to arrest A549 cancer cell growth at G1 phase in cell cycle test. We also found that methanol extract of S. saponaria extract treated A549 cancer cell shows necrosis signals in double staining test.
To justify the responsible signal pathway of necrosis in A549 cancer cell treatment with methanol extract of S. saponaria, we performed the Western Blotting test. The downstream protein caspase-3 was shown no cleaved fragments in treated A549 cell. However, PARP, the key protein in apoptosis and necrosis, has been cleaved into fragments. In addition, there has no noticeable difference among treatment time intervals in ROS test. Therefore, the methanol extract of S. saponaria may induce the necrosis of A549 cell not via ROS pathway but via the PARP activation.
|
|---|
