Establishment of multiplication protocols on medicinal plants Petasites formosanus Kitam. and Dysosma pleiantha (Hance) Woodson

• Main Authors: Hui-Ju Chang, 張慧如
• Other Authors: 廖松淵
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/12363641501788695673

碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 101 === Botanical drugs are mainly applied by the traditional Chinese medical science for thousands of years, it is passed on and never-ending. The medical science pays much attention to the combined therapy with Chinese and Western medicine gradually since developed, and focuses on medical plants. The aims of this study were to establish in vitro multiplication protocol of Taiwan native species, Petasites formosanus Kitam. and Dysosma pleiantha (Hance) Woodson. The processes include establishing aseptic explants,inducing shoots,rooting and transplanting. In the inducing shoots test, the test of Petasites formosanus Kitam. were BA 0.2 mg·L-1＋NAA 0.02 mg·L-1,BA 0.4 mg·L-1＋NAA 0.04 mg·L-1,BA 0.6 mg·L-1＋NAA 0.06 mg·L-1,BA 0.8 mg·L-1＋NAA 0.08 mg·L-1, with BA-NAA ratio 10:1 and the Murashige and Skoog (MS) medium was the control group.In vitro micropropagation of Petasites formosanus Kitam. was established to get maximum shoots multiplication rate by cultivating axillary buds on MS supplemented with BA 0.2 mg·L-1+ NAA 0.02 mg·L-1,but added plant growth regulators suppressing growth of explants and cause variation. In the rooting test, the test of Petasites formosanus Kitam. were MS, MS with 0.2 mg·L-1 IBA, MS with 0.5 mg·L-1 IBA ,1/2 MS, 1/2 MS with 0.2 mg·L-1 IBA, 1/2MS with 0.5 mg·L-1 IBA. Rooting rate achieved 100% by cultivating on MS plant medium for 2 weeks. 1/2MS with 0.5 mg·L-1 IBA has the most roots. Dysosma pleiantha (Hance) Woodson plant got the callus after being cultivated with different media and growth regulators, in which MS media with BA 2.0 mg·L-1+TDZ 0.2 mg·L-1induction of callus can continuously proliferate without browning and having died back, later generations to study.