Studies on the model of Cl- excretion in gills of tropical marine teleosts

• Main Authors: Tzu-Chen Lin, 林資宸
• Other Authors: Tsung-Hang Lee
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/40175396259729090042

碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 101 === Abstract Previous studies used euryhaline teleosts to illustrate the ionoregulatory model in seawater. Various tropical marine telesosts are famous and beautiful species in global aquaria. They are stenohaline species residing to hyperosmotic environments. In the southern marine of Taiwan, there are numerous species. However, few studies focused on the ionregulatory mechanism of the marine fishes to date. The present study selected 8 species to demonstrate the Cl- excretion of gill ionocytes. Two markers of the Cl- excretion, Na+, K+-ATPase (NKA) and Na+, K+, 2Cl- cotransporter 1 (NKCC1), were detected in gills of these marine fishes by western blotting with the α5 and T4 antibodies, respectively. The immunostaining section revealed that the ionocytes located prominently to the interlamellae regions of gill filaments. In addition, the distributions of NKCC1 were found in the basolateral membranes of the gill ionocytes. These results indicated that the tropical marine teleosts conformed to the Cl- excretion-model for adapting to the seawater.