Anti-inflammatory and Antioxidant Activities of Dendropanax dentiger

• Main Authors: Wei-Ning Hsu, 許惟寗
• Other Authors: 王升陽
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/54474722211379478022

碩士 === 國立中興大學 === 森林學系所 === 101 === 14 species of Araliaceae plants grown in Taiwan were collected and studied on their antioxidant, anti-inflammation, anti adipogenesis and cytotoxic activities. The results indicated that the EtOH extracts of Eleutherococcus trifoliatus and Schefflera octophylla possesses the strongest antioxidant activity in DPPH scavenging assay, respectively；and the extract of Dendropanax dentiger is less active. Meanwhile, Aralia cordata possessed the strongest cytotoxicity among the examed plants. It exhibited potent cytotoxicity against human hepatoma and human breast cancer cell. As regarding to anti-inflammation activity, Dendropanax dentiger exhibited significant anti-inflammatory activity, which could totally inhibit the expression of NO production by microphages at the dosage of 20 μg/mL. One bioactivity compound named (9Z,16S)-16-hydroxy-9,17-octadecadiene-12,14-diynoic acid (HODA) was isolated from the leaves of Dendropanax dentiger by using bioactivity guided fractionation protocol. The structure of HODA was identified using spectral analysis. Meanwhile, our data indicated that HODA significantly inhibited the production of NO in LPS-induced RAW 264.7 murine macrophage cells. Consistent with these observations, the mRNA and protein expression levels of iNOS was also inhibited by HODA in a dose-dependent manner. HODA also reduced the translocation of NF-κB in to nuclear induced by LPS, which is associated with the prevention of the degradation of I-κB, and subsequently decreased p65/p50 protein levels in the nucleus. On the other hand, HODA enhanced Nrf-2 activation and its downstream antioxidant gene HO-1. The anti-inflammation compound, HODA, also revealed the antioxidant effect on RAW264.7 cell after alcohol-induced oxidative damage.