Studies of the microbicidal effect of Vespa venom and Chinese herbal extracts on clinical pathogens

• Main Authors: Ching-Lin Shyu, 徐慶霖
• Other Authors: 邱繡河
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/50146091617097997977

博士 === 國立中興大學 === 微生物暨公共衛生學研究所 === 101 === The study is aimed to measure the antibacterial activity of polypeptide isolated from Vespa affinis venom and Chinese herbal extracts. The venom from V. affinis is a clear and light-yellow liquid with pH range 5 to 6. The protein concentration of the venom is 16.3 to 39.4 mg/ml. A strong antibacterial activity of vespa venom for gram positive and negative bacteria is founded by the Kirby-Bauer method. This venom can be separated as six fractions which are analyzed by using reverse phase High-performance liquid chromatography (HPLC), SDS-PAGE, and fast-atom- bombardment mass spectrometer, but the fraction 5 has antibacterial activity. The fraction 5 can be divided into the fraction 5-1 and the fraction 5-2 but only the fraction 5-1 with the molecular weight of 1512.96 Dalton, showed an antibacterial activity. After forth advanced analysis, the fraction 5-1 composed of 14 amino acids is classified as mastoparan-AF, the main composed of antibacterial compound from the V. affinis venom. Based on the chemical toxicity of shampoo used for pets, the second aim of this study is to evaluate the effects of herbal extracts on bacterial pathogens of clinical cases to evaluate a potential shampoo for the pet use. The antibacterial activity of crude extracts of 22 herbs were founded to be against 7 bacteria and 4 fungi. The therapeutic effects of crude alcoholic extracts of Inula japonica Thunb., Aconitum carmichaeli Debx. and A. kusnezoffii Reichb. on canine skin diseases were studied and an excellent antibacterial activity was founded compared with the control group. In conclusion, mastoparan-AF from the V. affinis venom and alcoholic extracts of I. japonica Thunb., A. carmichaeli Debx., and A. kusnezoffii Reichb. showed strong antibacterial activity. Finally, more study is needed to determine the structural of this protein and it’s bioactivity.